Single Molecule, Real-Time (SMRT®) long-read sequencing offers complete, uniform, nonbiased coverage spanning long amplicons. Unlike short-read technologies, SMRT sequencing does not require the amplicon to be fragmented, and allows for phasing of variants across the entire amplicon.
GENEWIZ offers ultra-deep, contiguous amplicon sequencing for sequences up to 10,000 bp. A single PacBio® read can discover a wide range of variants, from SNPs and small indels to large insertions and deletions. Furthermore, circular consensus sequences can be generated to obtain up to 99.999% single-molecule base calling accuracy.
Review our best practices when preparing amplicon DNA for PacBio sequencing.
GENEWIZ accepts a wide range of inputs for PacBio amplicon sequencing, including purified and unpurified amplicons, restriction digested material, and plasmids. Additionally, GENEWIZ can prepare an amplicon from a wide range of starting materials, including genomic DNA, cell pellets, and tissue material. For detailed sample submission requirements please visit our Sample Submission Guidelines.
Project deliverables include a data summary report, raw data files in BAM format, and demultiplex CCS reads in FASTQ format. Customizable data analysis packages are available by request.