Next Generation Sequencing: Amplicon-EZ

 

Amplicon sequencing made easy!

  • Easy on your budget – as low as $58 per sample
  • Easy to order – checkout online in minutes
  • Easy to submit samples – use a Azenta Life Sciences, formerly GENEWIZ drop box
  • Easy to analyze data – interactive reports with intuitive design
  • Easy on your deadline – results in 8 business days*

Amplicon-EZ is a streamlined version of next generation sequencing to analyze heterogeneous PCR products. Our standardized workflow simplifies ordering, processing, and analysis to deliver high-quality results fast.

 

 

Amplicon Sequencing in 8 Days*

1. Sample submission

  • No minimum sample number
  • Convenient sample submission via dropboxes

2. Library Preparation

  • 150-500 bp products accepted
  • Submit with or without lllumina® partial adapters

3. Sequencing

  • -50,000 reads per sample
  • Full sequence coverage with 2x2S0 bp sequencing

4. Data Analysis

  • Interactive variant report
  • Unique sequence identification and abundance analysis

*Samples are processed at our New Jersey, USA facility in cycles starting on Wednesdays with data returned 8 business days later (on the second Monday).

Applications: CRISPR SCREENING & MORE

CRISPR screening and validation – on/off-target mutations
Somatic variant analysis
Taxonomic identification – 16S, 18S, or ITS regions
Antibody discovery – variable regions of mAb, Fab, scFv, or sdAb genes
Clone verification and screening
Diversity analysis – synthetic DNA libraries

CRISPR Screening with Amplicon-EZ

Digital Spatial Profiling Stains

1. Edit cells with CRISPR

 

 

 
Digital Spatial Profiling Target Areas

2. Amplify target region from edited cell populations

 

 
Photocleave Barcodes

3. Use Amplicon-EZ to expedite sequencing of PCR products

 

 
Separate ROIs

4. Analyze results to find wells with desired mutations**

CRISPR Validation with Amplicon-EZ

 
Digital Spatial Profiling Stains

1. Isolate clones from postitive wells (see CRISPR screening)

 
Digital Spatial Profiling Target Areas

2. Amplify target region from clones

 
Photocleave Barcodes

3. Use Amplicon-EZ to expedite sequencing of PCR products

 
Separate ROIs

4. Validate edits and find homozygous mutants**

 

**View our interactive report (login required)


Want Azenta to perform amplification and sequencing? Learn about our additional NGS-based CRISPR validation services.

How To Order


Email | Phone 1-877-436-3949, Ext. 1