Amplicon-EZ


NGS-based amplicon sequencing offers detection of low frequency variants, quantitative analysis of mixed populations, and scalable analysis of a large number of samples. GENEWIZ’s Amplicon-EZ builds on the advantages of NGS-based screening and provides a cost-effective, fast, and interactive solution to help scientists expedite their research needs.

Amplicon-EZ is designed for purified PCR products with an insert size of 150-550bp*. Customers receive a guaranteed 50,000 reads per sample, delivery in as few as three business days, and an interactive data analysis report, at the lowest cost per sample.


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Applications

Synthetic/natural antibody repertoire screening
Somatic/complex variant analysis
16S/18S taxa analysis
Genome editing clone analysis

Features & Benefits

≥50,000 reads per sample
No minimum sample number requirements
Interactive analysis report available
Flexibility - Amplicons accepted with or without partial adapter sequences
Most cost-effective option

Project Workflow

Results in as few as three days**

Step 1

Submit Samples

Step 2

Library Generation

Step 3

Sequencing

Step 4

Data Reported



** Business days. Qualifying amplicons must be received at our NJ sequencing facility by Wednesday to be processed in that week's sequencing cycle. For San Francisco drop box users, please submit orders by Monday to meet this cutoff.

Sample Submission Guidelines

GENEWIZ accepts pre-made, purified amplicons either with or without Illumina partial overhangs. Insert size must be between 150-550bp* (not including partial overhangs). Amplicons must be received by Wednesday for same-week processing. For detailed sample submission requirements please visit our
Sample Submission Guidelines.

*Please note that our Amplicon-EZ offering sequences 150 bp from each end of your amplicon. The middle portion of amplicons >250bp will not be fully sequenced via the Amplicon-EZ offering. For full length sequencing of middle regions on amplicons >250bp, please refer to our standard amplicon sequencing service page.


Deliverables

Standard Available
  • Raw data delivered via email
  • Free post-delivery support
  • Variant analysis and/or clone verification via interactive data analysis platform
  • Unique sequence identification and abundance analysis
  • Custom assembly

How To Order


Email | Phone (1-877-GENEWIZ (436-3949), Ext. 1) | Live Chat