Codon Optimization


As experts in synthetic DNA, we understand the importance of accurate gene synthesis and its subsequent expression, particularly in heterologous systems. A multitude of factors, including codon bias, can affect the transcriptional and translational efficiency of a gene in a host organism. Azenta Life Sciences, formerly GENEWIZ’s codon optimization tool considers these critical factors to produce codon optimized gene sequences for efficient expression in almost all host systems. 


Codon Optimized Synthetic GENES

Codons represent the genetic code that transfers information from genes to mRNA to protein. Both redundancy and evolutionary constraints, including the availability of tRNA isoacceptors, TATA box, Shine-Dalgarno sequences, and more, result in preferential usage of one codon over another for the same amino acid. This phenomenon is known as codon bias.

Codon optimization is a process used to improve gene expression and increase the translational efficiency of a gene of interest by accommodating codon bias of the host organism.

Download your complimentary copy of our tech note, Enhancing Protein Expression by Leveraging Codon Optimization, to learn how you can leverage Azenta’s free codon optimization tool to enhance your protein expression.

Protein Expression

Schematic representation of protein expression

Tech Note: Enhancing Protein Expression by Leveraging Codon Optimization


Optimize and Order In Minutes

The introduction of optimization algorithms, a type of artificial intelligence, has significantly streamlined the process of designing a DNA sequence to optimize protein expression in target organisms. When you partner with Azenta, you get access to our free codon optimization algorithm tool that recognizes and optimizes key parameters to stabilize DNA sequences and improve gene expression. Choose from dozens of expression systems and hundreds of restriction enzyme sites while we ensure that your sequence is tailored to your research project.

High protein expression – Azenta’s algorithm analyzes a wide range of data to optimize critical parameters and significantly improve protein expression levels.
Difficult sequence normalization – Our codon optimization tool can normalize difficult sequences to remove unfavorable regions and reduce local GC content to desired percentages.
Comprehensive codon usage tables – Species-specific codon usage tables allow the tool to identify and replace low-frequency codons with high-frequency codons for all major host organisms.
Ph.D.-level expertise and support – Our dedicated team of scientists provide consultations and support for all your experimental needs, from start to finish.

Related Services


Over 99.9% Delivery Rate Even on Complex Projects

Backed by our 99.9% completion rate, Azenta synthesizes naturally occurring sequences of any length or complexity for your protein and antibody engineering, CRISPR gene editing, and more. Rather than spending your valuable time on DNA cloning, Azenta will custom clone your codon-optimized genes into your vector for optimal protein expression. We also offer a wide range of synthetic DNA solutions for your research, including gene fragment synthesis, synthetic DNA libraries, oligo synthesis, and CRISPR synthetic solutions.

Azenta’s streamlined processes and protocols allow us to deliver timely and dependable results with proactive Ph.D.-level project support every step of the way. It's no wonder top research labs, pharmaceutical, and biotechnology companies around the world rely on Azenta for all of their synthetic DNA needs.


*Samples must arrive at the GENEWIZ New Jersey Laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.

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