Primer walking: service options

Primer Walking: Sequence Discovery

• Plasmid - Submit 1 µg per kb of target sequence for single-stranded coverage, 2 µg per kb sequence for double-stranded coverage, and 4 µg per kb for 4x coverage. Submit at least 10 µg plasmid if region of interest is of unknown length. Please submit a minimum concentration of 200 ng/I per sample.

• PCR Products - Submit 10-40 ng per kb of amplicon.

• ITRs - For plasmids and PCR products that contain ITRs, please submit an additional 1 µg of material per ITR in the sample sequence.

• Primers - GENEWIZ can synthesize the initial primer if the sequence for a known region (e.g. antibiotic resistance) is provided. If you have primers that can be used in the initial round of sequencing, please submit a minimum of 10 µl each at 5 µM.

• Packaging - For DNA and primers, please use 1.5 ml screw cap tubes or microcentrifuge tubes secured with parafilm and label them with sample or primer name and concentration.

 

Primer Walking: Sequence Confirmation

• Plasmid - Submit 1 µg per kb of target sequence for single-stranded coverage, 2 µg per kb sequence for double-stranded coverage, and 4 µg per kb for 4x coverage. Please submit a minimum concentration of 200 ng/µl per sample.

• PCR Products - Submit 10-40 ng per kb of amplicon.

• ITRs - For plasmids and PCR products that contain ITRs, please submit an additional 1 µg of material per ITR in the sample sequence.

• Primers - GENEWIZ can synthesize all of the primers if the known reference sequence is provided. If you have primers that can be used in the initial round of sequencing, please submit a minimum of 10 µl each at 5 µM.

• Packaging - For DNA and primers, please use 1.5 ml screw cap tubes or microcentrifuge tubes secured with parafilm and label them with sample or primer name and concentration.

 

Shipping

US - Send samples to GENEWIZ NJ via standard overnight service

Ship to:
Attn: Molecular Genetics - PW
115 Corporate Blvd
South Plainfield, NJ 07080
USA

 

OR contact us at primerwalking@genewiz.com to find out if there is a GENEWIZ Drop Box or courier pickup service available for your location.