Next Generation Sequencing
View our Sample Submission Best Practices Checklist .
Interactive Sample Submission Guidelines
Direct Shipping (FedEx, UPS):
Please use an overnight delivery service such as FedEx or UPS for all NGS samples. A print out of the confirmed quote must be included with the material.
All direct shipments should be sent to:
GENEWIZ
Attn: NGS
111 Corporate Boulevard
Suite H Loading Dock
South Plainfield, NJ 07080
USA
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DNA Sample Submission Guidelines
DNA can be concentrated free of charge via SpeedVacTM . Additional charges will apply for concentration via column or other methods.
Whole Genome Sequencing / Low-Pass WGS / Shotgun Sequencing
Sample Type : Genomic DNA
Sample Purity (OD260/280): 1.8-2.0
Recommended Quantity: >1.5 µg , >20 ng/µ l
Minimum Amount: 500 ng
Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Shipment Method: Dry Ice
Single-Cell ATAC-Seq
Sample Type: Cryopreserved single-cell suspension according to GENEWIZ-supplied protocol, submitted in duplicate
Recommended Quantity: >1M cells in 1 mL
Minimum Amount: 100,000 cells in 500 µL (Note: 1E6 cells are required for dead cell removal)
Buffer: Cryopreservation media (e.g. cell culture media supplemented with 20% FBS and 10% DMSO)
Shipment Method: Dry Ice
ChIP-Seq Sample Submission Guidelines
Sample Type: ChIP DNA
Sample Purity (OD 260/280): 1.8-2.0
Recommended Quantity: >50 ng
Minimum Amount: 10 ng
DNA Fragment Length: 100-800 bp; 200-300 bp (Main peak)
Resuspension Buffer: Water, BE, or low TE (<0.1 mM EDTA)
Shipment Method: Dry Ice
Agilent SureSelect Exome Enrichment Kit, OncoGxOne™ Discovery Cancer Panels and TargetGxOne™ Gene Enrichment
Sample Type: Genomic DNA
Sample Purity (OD260/280): 1.8-2.0
Recommended Quantity: >1.5 µg, >20 ng/µl
Minimum Amount: 500 ng
Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Shipment Method: Dry Ice
Custom TargetGxOne™ Amplicon Sequencing
Sample Type: Genomic DNA
Sample Purity (OD260/280): 1.8-2.0
Recommended Quantity: >1 µg, >20 ng/µl
Minimum Amount: 100 ng
Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Shipment Method: Dry Ice
16S MetaVx™ Panels/ITS 2
Sample Type: Genomic DNA
Sample Purity (OD260/280): 1.8-2.0
Recommended Quantity: >1 µg, >20 ng/µl
Minimum Amount: 100 ng
Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Shipment Method: Dry Ice
Pre-made Sequencing Libraries
Recommended Quantity: >15 nM
Volume: ≥20 ul
Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Shipment Method: Dry Ice
genoTYPER-NEXT
Sample Type (Preferred): Fresh/Frozen Cell Pellet
Recommended Quantity: ≥106 cells
Required Quantity: ≥3x104 cells
Shipment Method: Dry Ice
Sample Type: Genomic DNA
Sample Purity (OD260/280): 1.8-2.0, RNA-Free
Recommended Quantity: >1.5µg, >20 ng/µl
Minimum Amount: 500 ng
Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Shipment Method: Dry Ice
Amplicon-EZ
Sample Type: Purified PCR Products
Sample Purity (OD260/280): 1.8-2.0
Concentration: 20 ng/µl
Minimum Amount: 500 ng
Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
Shipment Method: Room Temperature/Blue Ice/Dry Ice
RNA Sample Submission Guidelines
We now offer RNA Stabilization Tubes. Ship RNA safely at ambient temperature. Download the information sheet. Contact us to receive a free trial.
Standard/Strand-Specific RNA-Seq & Immuno-Profiling
Sample Type: Total RNA
Sample Purity (OD260/OD280): 1.8-2.2
Recommended RIN: ≥6.0
Recommended Quantity: >2 µg, >50 ng/µl
Minimum Quantity: 500 ng
Resuspension Buffer: Nuclease-free water
Shipment Method: Dry Ice
Small RNA-Seq
Sample Type: Total RNA (containing small RNA) in nuclease-free water, on dry ice
Sample Purity (OD260/OD280): 1.8-2.2, DNA-free, ≥ 50 ng/μL, 50 ng/μL
Recommended RIN: ≥6.0
Recommended Quantity: Total RNA: ≥2 µg, >50 ng/µl, Purified Small RNA: >50 ng, >5 ng/µl
Resuspension Buffer: Nuclease-free water
Shipment Method: Dry Ice
Targeted RNA-Seq
Sample Type: Total RNA
Sample Purity (OD260/OD280): 1.8-2.2
Recommended RIN: ≥6.0
Recommended Quantity: >2 µg, >50 ng/µl
Minimum Quantity: 1 µg
Resuspension Buffer: Nuclease-free water
Shipment Method: Dry Ice
Ultra-Low Input RNA-Seq
Cell number and buffer clearly indicated in GENEWIZ Sample Submission Form
Sample Type: Cells in solution or small amounts of total RNA
Recommended Quantity: >1,000 cells/sample in Trizol or 20 ng of total RNA
Shipment Method: Dry Ice
Single-Cell RNA-Seq & Single-Cell Immuno-Profiling
Sample Type: Frozen cell suspension according to GENEWIZ-supplied protocol, submitted in duplicate. Methanol-fixed cells are also accepted.
Sample Viability: >90% post-thaw
Recommended Quantity: >1M cells in 1 mL
Minimum Quantity: 50,000 cells in 500 µL
Resuspension Buffer: Appropriate cryopreservation media (e.g., cell culture media supplemented with 20% FBS and 10% DMSO)
Shipment Method: Dry Ice overnight
PacBio Iso-Seq
Sample Type: Total RNA
Sample Purity(OD260/280): 1.8 - 2.2
Recommended RIN: ≥9.0
Recommended Quantity: ≥2µg
Recommended Volume: 10 - 20µL
Shipment Method: Dry Ice
All extractions are performed to best of GENEWIZ’s ability and the Client is charged for work performed regardless of outcome. Additional charges for procurement of kits may apply for select extractions. Please Note: we do not accept samples above BSL 2 and we require prior notification for pathogenic samples.
FFPE Samples
Recommended Quantity: ≥4 FFPE slides recommended
Minimum Quantity: 2 FFPE slides
Thickness: 5µm-20 µm
Area: >150 mm2
Shipping Method: Room Temperature/Blue Ice
Cell pellets
Recommended Quantity: 106 cells
Minimum Quantity: 104 cells
Shipping Method: Dry Ice
Fresh Frozen Tissue
Recommended Quantity: 10 mg
Minimum Quantity: 2 mg
Shipping Method: Dry Ice
Viral Particles
Recommended Quantity: >1 mL supernatant
Viral Titer: >5x109 cells/mL
Shipping Method: Dry Ice
Whole Blood
Recommended Quantity: 2 tubes/sample
Recommended Volume (DNA Extraction): 500 µl/tube
Minimum Volume (DNA Extraction): 200 µl/tube
Recommended Volume (RNA Extraction): 2.5 mL (whole blood, PAXgene Blood RNA Tube)
Shipping Method: Fresh: Blue Ice, Frozen: Dry Ice
Plasma/Serum
Recommended Quantity: 2 tubes/sample
Recommended Volume: 10 mL/tube
Minimum Volume: 5 mL/tube
Shipping Method: Dry Ice
Stool
Recommended Quantity: 100 mg
Minimum Quantity: 50 mg
Shipping Method: Dry Ice/Blue Ice
Saliva
Recommended Quantity: 1 mL
Shipping Method: Dry Ice/Blue Ice
Soil
Recommended Quantity: 100 mg
Minimum Quantity: 50 mg
Shipping Method: Room Temperature/Blue Ice
Water
Recommended Volume: 100 mL
Minimum Volume: 20 mL
Shipping Method: Room Temperature
Swab
Recommended Quantity: 2 tubes/sample. 1 swap/tube
Shipping Method: Room Temperature
Fixed Needle Core Biopsy
Recommended Quantity: 4 biopsies
Minimum Quantity: 1 biopsy
Recommended Diameter: 1-2 mm diameter; >5 mm in length
Shipping Method: Room Temperature/Blue Ice
Snap Frozen Needle Core Biopsy
Recommended Quantity: 10 mg
Minimum Quantity: 3 mg
Shipping Method: Dry Ice
GENEWIZ Sample Quality Control Assessment
GENEWIZ performs QC free of charge with results reported within 24 – 48 hours.
DNA*
RNA
Pre-Made Libraries
TapeStation
Qubit Assay
*For High Molecular Weight DNA, gel electrophoresis will also be performed.
For questions on submitting other, or less than ideal sample types, please contact our Project Management team to discuss further as we may be able to proceed with your project.
Phone: +1 (908) 222-0711 ext. 1
Email: NGS@genewiz.com