DNA Sample Submission Guidelines

Whole Genome Sequencing / Low-Pass WGS / Shotgun Sequencing

• Sample Type: Genomic DNA
• Sample Purity (OD260/280): 1.8-2.0
• Recommended Quantity: >1.5 µg, >20 ng/µl
• Minimum Amount: 500 ng
• Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry Ice

Single-Cell ATAC-Seq

• Sample Type: Cryopreserved single-cell suspension according to Azenta-supplied protocol, submitted in duplicate
• Recommended Quantity: >1M cells in 1 mL
• Minimum Amount: 100,000 cells in 500 µL (Note: 1E6 cells are required for dead cell removal)
• Buffer: Cryopreservation media (e.g. cell culture media supplemented with 20% FBS and 10% DMSO)
• Shipment Method: Dry Ice

ChIP-Seq

• Sample Type: ChIP DNA
• Sample Purity (OD 260/280): 1.8-2.0
• Recommended Quantity: >50 ng
• Minimum Amount: 10 ng
• DNA Fragment Length: 100-800 bp; 200-300 bp (Main peak)
• Resuspension Buffer: Water, BE, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry Ice

Agilent SureSelect Exome Enrichment Kit, OncoGxOne™ Discovery Cancer Panels and TargetGxOne™ Gene Enrichment

• Sample Type: Genomic DNA
• Sample Purity (OD260/280): 1.8-2.0
• Recommended Quantity: >1.5 µg, >20 ng/µl
• Minimum Amount: 500 ng
• Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry ice

Custom TargetGxOne™ Amplicon Sequencing

• Sample Type: Genomic DNA
• Sample Purity (OD260/280): 1.8-2.0
• Recommended Quantity: >1 µg, >20 ng/µl
• Minimum Amount: 100 ng
• Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry ice

16S MetaVx™ Panels/ITS 2

• Sample Type: Genomic DNA
• Sample Purity (OD260/280): 1.8-2.0
• Recommended Quantity: >1 µg, >20 ng/µl
• Minimum Amount: 100 ng
• Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry ice

Pre-Made Sequencing Libraries

• Recommended Quantity: >15 nM
• Volume: ≥20 ul
• Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry ice

genoTYPER-NEXT

• Sample Type (Preferred): Fresh/frozen cell pellet
• Recommended Quantity: ≥10⁶ cells
• Required Quantity: ≥3x10⁴ cells
• Shipment Method: Dry ice

• Sample Type: Genomic DNA
• Sample Purity (OD260/280): 1.8-2.0, RNA-free
• Recommended Quantity: >1.5µg, >20 ng/µl
• Minimum Amount: 500 ng
• Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry ice

Amplicon-EZ

• Sample Type: Purified PCR products
• Sample Purity (OD260/280): 1.8-2.0
• Concentration: 20 ng/µl
• Minimum Amount: 500 ng
• Resuspension Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Room temperature/blue ice/dry ice

RNA Sample Submission Guidelines

We now offer RNA Stabilization Tubes. Ship RNA safely at ambient temperature. Download the information sheet. Contact us to receive a free trial.

Standard/Strand-Specific RNA-Seq & Immuno-Profiling

• Sample Type: Total RNA
• Sample Purity (OD260/OD280): 1.8-2.2
• Recommended RIN: ≥6.0
• Recommended Quantity: >2 µg, >50 ng/µl
• Minimum Quantity: 500 ng
• Resuspension Buffer: Nuclease-free water
• Shipment Method: Dry ice

Small RNA-Seq

• Sample Type: Total RNA (containing small RNA) in nuclease-free water, on dry ice
• Sample Purity (OD260/OD280): 1.8-2.2, DNA-free, ≥ 50 ng/μL, 50 ng/μL
• Recommended RIN: ≥6.0
• Recommended Quantity: Total RNA: ≥2 µg, >50 ng/µl, purified small RNA: >50 ng, >5 ng/µl
• Resuspension Buffer: Nuclease-free water
• Shipment Method: Dry ice

Ultra-Low Input RNA-Seq

Cell number and buffer clearly indicated in Azenta Sample Submission Form

• Sample Type: Cells in solution or small amounts of total RNA
• Recommended Quantity: >1,000 cells/sample in Trizol or 20 ng of total RNA
• Shipment Method: Dry ice

Single-Cell RNA-Seq & Single-Cell Immuno-Profiling

• Sample Type: Frozen cell suspension according to Azenta-supplied protocol, submitted in duplicate. Methanol-fixed cells are also accepted.
• Sample Viability: >90% post-thaw
• Recommended Quantity: >1M cells in 1 mL
• Minimum Quantity: 50,000 cells in 500 µL
• Resuspension Buffer: Appropriate cryopreservation media (e.g., cell culture media supplemented with 20% FBS and 10% DMSO)
• Shipment Method: Dry ice overnight

High-Throughput Gene Expression (HT-GEx) Screening

• Recommended Quantity: Frozen cell lysates, 5000 - 20,000 cells in 15µl of lysis buffer
• Recommended Lysis Buffer: QIASeq UPX Cell Lysis Buffer (#333076) or equivalent
• Shipment Method: Dry ice
• Sample Container: PCR-clean 96-well PCR-plate -80°C dry-ice resistant and foil sealed
• Sample Type:
• Cell lysate
• Purified RNA (please contact us for details)

PacBio Iso-Seq

• Sample Type: Total RNA
• Sample Purity (OD260/280): 1.8 - 2.2
• Recommended RIN: ≥9.0
• Recommended Quantity: ≥2µg
• Recommended Volume: 10 - 20µL
• Shipment Method: Dry ice

Extraction Requirements

FFPE Samples

• Recommended Quantity: ≥4 FFPE slides recommended
• Minimum Quantity: 2 FFPE slides
• Thickness: 5µm-20 µm
• Area: >150 mm²
• Shipping Method: Room temperature/blue ice

Cell pellets

• Recommended Quantity: 10⁶ cells
• Minimum Quantity: 10⁴ cells
• Shipping Method: Dry ice

Fresh Frozen Tissue

• Recommended Quantity: 10 mg
• Minimum Quantity: 2 mg
• Shipping Method: Dry ice

Viral Particles

• Recommended Quantity: >1 mL supernatant
• Viral Titer: >5x10⁹ cells/mL
• Shipping Method: Dry ice

Whole Blood

• Recommended Quantity: 2 tubes/sample
• Recommended Volume (DNA Extraction): 500 µl/tube
• Minimum Volume (DNA Extraction): 200 µl/tube
• Recommended Volume (RNA Extraction): 2.5 mL (whole blood, PAXgene Blood RNA Tube)
• Shipping Method: Fresh: Blue ice, frozen: Dry ice

Plasma/Serum

• Recommended Quantity: 2 tubes/sample
• Recommended Volume: 10 mL/tube
• Minimum Volume: 5 mL/tube
• Shipping Method: Dry ice

Stool

• Recommended Quantity: 100 mg
• Minimum Quantity: 50 mg
• Shipping Method: Dry ice/blue ice

Saliva

• Recommended Quantity: 1 mL
• Shipping Method: Dry ice/blue ice

Soil

• Recommended Quantity: 100 mg
• Minimum Quantity: 50 mg
• Shipping Method: Room temperature/Blue Ice

Water

• Recommended Volume: 100 mL
• Minimum Volume: 20 mL
• Shipping Method: Room temperature

Swab

• Recommended Quantity: 2 tubes/sample. 1 swap/tube
• Shipping Method: Room temperature

Fixed Needle Core Biopsy

• Recommended Quantity: 4 biopsies
• Minimum Quantity: 1 biopsy
• Recommended Diameter: 1-2 mm diameter; >5 mm in length
• Shipping Method: Room temperature/blue ice

Snap Frozen Needle Core Biopsy

• Recommended Quantity: 10 mg
• Minimum Quantity: 3 mg
• Shipping Method: Dry ice

Azenta Sample Quality Control Assessment

Azenta performs QC free of charge with results reported within 24 – 48 hours.

*For High Molecular Weight DNA, gel electrophoresis will also be performed.

For questions on submitting other, or less than ideal sample types, please contact our Project Management team to discuss further as we may be able to proceed with your project.

Phone: (877) 436-3949 ext. 1

Email: NGS@azenta.com