DNA Sample Submission Guidelines

Whole Exome Sequencing (CLIA-Validated Service)

• Sample Type: Genomic DNA
• Recommended Quantity: ≥1 µg
• Required Quantity: ≥600 ng
• Concentration: ≥20 ng/µL
• Purity: A260/280 = 1.6-2.2
• Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry Ice

• Sample Type: Whole blood in lavender tube
• Recommended Quantity: 5 mL
• Required Quantity: 2 mL
• Shipping Method: Dry Ice

Whole Exome Sequencing (CLIA Environment)

• Sample Type: Genomic DNA
• Recommended Quantity: ≥1 µg
• Required Quantity: ≥600 ng
• Concentration: ≥20 ng/µL
• Purity: A260/280 = 1.6-2.2
• Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry Ice

• Sample Type: Whole blood in lavender tube
• Recommended Quantity: 5 mL
• Required Quantity: 2 mL
• Shipping Method: Dry Ice

• Sample Type: Fresh frozen tissue
• Recommended Quantity: ≥20 mg
• Required Quantity: ≥10 mg
• Shipping Method: Dry Ice

• Sample Type: Saliva in Oragene collection tube
• Required Quantity: 2 mL
• Shipping Method: Room temperature

• Sample Type: FFPE slides
• Recommended Quantity: ≥4 slides
• Required Quantity: ≥2 slides
• Thickness: 5-20 µm
• Area: >150 mm²
• Shipment Method: Room Temperature

Whole Genome Sequencing (CLIA-Validated Service)

• Sample Type: Genomic DNA
• Recommended Quantity: ≥2 µg
• Required Quantity: ≥1.1 µg
• Concentration: ≥20 ng/µL
• Purity: A260/280 = 1.6-2.2
• Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry Ice

• Sample Type: Whole blood in lavender tube
• Recommended Quantity: 5 mL
• Required Quantity: 2 mL
• Shipping Method: Dry Ice

Whole Genome Sequencing (CLIA Environment)

• Sample Type: Genomic DNA
• Recommended Quantity: ≥2 µg
• Required Quantity: ≥1.1 µg
• Concentration: ≥20 ng/µL
• Purity: A260/280 = 1.6-2.2
• Buffer: Water, EB, or low TE (<0.1 mM EDTA)
• Shipment Method: Dry Ice

• Sample Type: Whole blood in lavender tube
• Recommended Quantity: 5 mL
• Required Quantity: 2 mL
• Shipping Method: Dry Ice

• Sample Type: Fresh frozen tissue
• Recommended Quantity: ≥20 mg
• Required Quantity: ≥10 mg
• Shipping Method: Dry Ice

• Sample Type: Saliva in Oragene collection tube
• Required Quantity: 2 mL
• Shipping Method: Room temperature

• Sample Type: FFPE slides
• Recommended Quantity: ≥4 slides
• Required Quantity: ≥2 slides
• Thickness: 5-20 µm
• Area: >150 mm²
• Shipment Method: Room Temperature

For questions on submitting other, or less than ideal sample types, please contact our Project Management team to discuss further as we may be able to proceed with your project.

Phone: +1 (908) 222-0711 ext. 1

Email: clia@azenta.com