Highlights

• TransStart® Taq DNA Polymerase offers 18-fold fidelity as compared to EasyTaq®DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Reduced nonspecific amplification and primer dimer formation.
• Different from Taq antibody, no risk of contamination from mammalian DNA.
• Different from chemical modification, long denaturing step is not needed.
• Amplification of genomic DNA fragment up to 15 kb.

Applications

• Complex templates
• GC/AT-rich templates
• Multiplex PCR
• High yield PCR

Unit Definition

One unit of TransStart®Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74˚C.

Quality Control

TransStart® Taq DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch ofTransStart®Taq DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers

Storage

-20˚C for two years

Shipping Condition

Dry ice (-70˚C)

Component

AP141-01/11

AP141-02/12

AP141-03/13

TransStart® Taq DNA Polymerase

250 U×1

500 U × 1

500 U × 6

10× TransStart®Taq Buffer

1.2 ml

1.2 ml × 2

1.2 ml × 12

2.5 mM dNTPs

-/800 μl × 1

-/800 μl × 2

-/1.2 ml × 8

10x GC Enhancer

200 μl × 1

400 μl × 1

1ml x 1

6x DNA Loading Buffer

500 μl × 1

1ml x 1

1ml x 2