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TransStart® Taq DNA Polymerase (with 2.5 mM dNTPs)

TransStart® Taq DNA Polymerase is a novel hot-start enzyme. It uses two proprietary DNA binding proteins that at room temperature bind to the double-strand DNA template and primer, effectively neutralizing the DNA polymerase activity until denaturation occurs. As the denaturation step proceeds, the two proteins are inactivated, and the released primers and templates participate in the amplification reaction, enhancing PCR amplification efficiency.

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Highlights

• TransStart® Taq DNA Polymerase offers 18-fold fidelity as compared to EasyTaq®DNA Polymerase.
• Extension rate is about 1-2 kb/min.
• Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors.
• Reduced nonspecific amplification and primer dimer formation.
• Different from Taq antibody, no risk of contamination from mammalian DNA.
• Different from chemical modification, long denaturing step is not needed.
• Amplification of genomic DNA fragment up to 15 kb.

Applications

• Complex templates
• GC/AT-rich templates
• Multiplex PCR
• High yield PCR

Unit Definition

One unit of TransStart®Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74˚C.

Quality Control

TransStart® Taq DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch ofTransStart®Taq DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA.

Storage Buffer

20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers

Storage

-20˚C for two years

Shipping Condition

Dry ice (-70˚C)

 

Kit Contents

Component

AP141-01/11

AP141-02/12

AP141-03/13

TransStart® Taq DNA Polymerase

250 U×1

500 U × 1

500 U × 6

10× TransStart®Taq Buffer

1.2 ml

1.2 ml × 2

1.2 ml × 12

2.5 mM dNTPs

-/800 μl × 1

-/800 μl × 2

-/1.2 ml × 8

10x GC Enhancer

200 μl × 1

400 μl × 1

1ml x 1

6x DNA Loading Buffer

500 μl × 1

1ml x 1

1ml x 2