Highlights |
• A combination of chemical blocking technique with TransStart® hot start technique to achieve complete blocking. Compared with double blocking TransStart® TopTaq, this method provides higher sensitivity, higher specificity and better amplification. |
Passive Reference Dye |
• Passive Reference Dye I (50×): ABI Prism7000/7300/7700/7900, Eppendorf, ABI Step One, ABI Step One Plus |
Storage |
-20˚C for one year |
Kit Contents
Component |
AQ141-01 |
AQ141-02 |
AQ141-03 |
AQ141-04 |
2×TransStart® Tip Green qPCR SuperMix |
1 ml |
5 × 1 ml |
15 × 1 ml |
25 × 1 ml |
Passive Reference Dye (50×) |
40 μl |
200 μl |
600 μl |
1 ml |
ddH2O |
1 ml |
5 ml |
3 × 5 ml |
5 × 5 ml |