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EasyPure® RNA Kit

EasyPure® RNA Kit

EasyPure® RNA Kit

EasyPure RNA Kit provides a simple and fast column based method to isolate total RNA from animal cells, animal tissues, bacteria and yeast. Cells and tissues are enzymatically lysed. DNA is digested with DNase I. RNA is bound to silica membrane. After washing, high quality RNA is eluted from the column.

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Applications	RT-PCR, qRT-PCR and Northern blot.
Storage	Proteinase K solution, DNase I and DNase I Reaction Buffer at -20°C for 1 yr; others at room temperature for 1 yr
Shipping Condition	Proteinase K and DNase I on dry ice (-70°C); others at room temperature

Kit Contents

Component	ER101-01 (50 rxns)
Binding buffer 4 (BB4)	40 ml
Clean buffer 4 (CB4)	60 ml
Wash buffer 4 (WB4)	12 ml
Proteinase K (20 mg/ml)	1 ml
DNase I (3 units/μl)	1500 units
DNase I Reaction Buffer	4 × 1 ml
RNase-free Water	10 ml
RNase-free Tube (1.5 ml)	50 each
RNA Spin Columns with Collection Tubes	50 each

EasyPure RNA Kit