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EasyPure® RNA Kit

EasyPure RNA Kit provides a simple and fast column based method to isolate total RNA from animal cells, animal tissues, bacteria and yeast. Cells and tissues are enzymatically lysed. DNA is digested with DNase I. RNA is bound to silica membrane. After washing, high quality RNA is eluted from the column.

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Applications

RT-PCR, qRT-PCR and Northern blot.

Storage

Proteinase K solution, DNase I and DNase I Reaction Buffer at -20°C for 1 yr; others at room temperature for 1 yr

Shipping Condition

Proteinase K and DNase I on dry ice (-70°C); others at room temperature

 

Kit Contents

Component

ER101-01 (50 rxns)

Binding buffer 4 (BB4)

40 ml

Clean buffer 4 (CB4)

60 ml

Wash buffer 4 (WB4)

12 ml

Proteinase K (20 mg/ml)

1 ml

DNase I (3 units/μl)

1500 units

DNase I Reaction Buffer

4 × 1 ml

RNase-free Water

10 ml

RNase-free Tube (1.5 ml)

50 each

RNA Spin Columns with Collection Tubes

50 each