Highlights |
• TransStart® Taq DNA Polymerase offers 18-fold fidelity as compared to EasyTaq®DNA Polymerase. |
Applications |
• Complex templates |
Unit Definition |
One unit of TransStart®Taq DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74˚C. |
Quality Control |
TransStart® Taq DNA Polymerase has passed the following quality control assays: functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. Each batch ofTransStart®Taq DNA Polymerase has been assayed for amplification efficiency to amplify p53 gene from 10 ng of human genomic DNA. |
Storage Buffer |
20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers |
Storage |
-20˚C for two years |
Shipping Condition |
Dry ice (-70˚C) |
Kit Contents
Component |
AP141-01/11 |
AP141-02/12 |
AP141-03/13 |
TransStart® Taq DNA Polymerase |
250 U×1 |
500 U × 1 |
500 U × 6 |
10× TransStart®Taq Buffer |
1.2 ml |
1.2 ml × 2 |
1.2 ml × 12 |
2.5 mM dNTPs |
-/800 μl × 1 |
-/800 μl × 2 |
-/1.2 ml × 8 |
10x GC Enhancer |
200 μl × 1 |
400 μl × 1 |
1ml x 1 |
6x DNA Loading Buffer |
500 μl × 1 |
1ml x 1 |
1ml x 2 |