Frequently Asked Questions
Can GENEWIZ optimize genes?
GENEWIZ can deliver codon-optimized genes, designed to meet your exact research specifications.
Can GENEWIZ synthesize sequences with high GC content or repeats?
GENEWIZ can synthesize difficult templates, such as GC-rich sequences and sequences with repeats,
using proprietary technologies. With our bioinformatics platform, GENEWIZ optimizes your gene for synthesis.
We carefully review every sequence and provide a no obligation quote for every project. Each custom quote includes estimated pricing, turnaround time, service description, and analysis results.
How do I know that I am getting the correct sequence?
GENEWIZ conducts sequence verification during the gene synthesis process to ensure your synthesis product is as intended.
Final plasmid constructs are clonally isolated and confirmed by Sanger DNA sequence analysis to at least single-strand depth across the synthesized insert.
Purified PCR fragments are sequence verified on both strands, although verification at the fragment ends is limited to single strand depth.
Subassemblies and intermediate products may be cloned and verified to contain the correct sequence, as needed.
DNA sequence re-verification after scale-up DNA preparation is available as an added option to your gene synthesis order.
On completion of your synthetic gene, GENEWIZ delivers an electronic data package in parallel with your construct.
The data package includes the original sequencing data files from construct verification to facilitate your independent review.
Why use GENEWIZ gene synthesis services?
Gene synthesis is an efficient and cost-effective alternative to molecular
cloning for custom gene production. When compared to PCR cloning, GENEWIZ gene
synthesis offers faster turnaround time for a comparable price. For a comparison
of GENEWIZ custom gene synthesis services versus traditional molecular cloning,
please click here.
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