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Frequently Asked Questions
Can GENEWIZ optimize genes?
GENEWIZ can deliver codon-optimized genes, designed to meet your exact research specifications.
Can GENEWIZ synthesize sequences with high GC content or repeats?
GENEWIZ can synthesize difficult templates, such as GC-rich sequences and sequences with repeats,
using proprietary technologies. With our bioinformatics platform, GENEWIZ optimizes your gene for synthesis.
We carefully review every sequence, and provide a custom quote for every project, which includes turnaround time and price details.
How do I know that I am getting the correct sequence?
GENEWIZ employs quality controls throughout the duration of all
gene synthesis projects to ensure accurate synthesis, starting with inclusion of
your exact sequence for synthesis within your custom gene synthesis quote. This
enables customers to review and confirm the sequence prior to project
initiation.
Once beginning your project, GENEWIZ conducts sequence
verification during the gene assembly process, verifying that any subassemblies
contain the correct sequence. Subsequently, the final plasmid DNA is clonally isolated and confirmed to match your sequence by Sanger DNA sequence analysis to at least single-strand depth.
Purified PCR fragments are sequence verified on both strands, although verification at the fragment ends is limited to single strand depth.
Upon completion of your synthetic gene, GENEWIZ delivers an electronic data package in parallel with your
construct, containing the original sequencing data files from construct
verification for your independent review.
Final deliverables for GENEWIZ gene synthesis projects include:
- Certificate of Analysis (COA), including a gel image of a restriction digest test (cloned vector products, only) or purified PCR fragment;
- Alignment of sequencing data with the complete insert sequence;
- The complete vector sequence, if applicable.
Why use GENEWIZ gene synthesis services?
Gene synthesis is an efficient and cost-effective alternative to molecular
cloning for custom gene production. When compared to PCR cloning, GENEWIZ gene
synthesis offers faster turnaround time for a comparable price. For a comparison
of GENEWIZ custom gene synthesis services versus traditional molecular cloning,
please click here.
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