Methylation Sequencing
Methylation sequencing (Methyl-Seq) is a technique used to quantify DNA methylation – an epigenetic modification that alters gene expression – by converting nucleotide bases using sodium bisulfite. It allows you to map and quantify the presence of methyl groups on DNA strands—a critical component of gene regulation and expression. In eukaryotes, cytosine often methylates to form 5-methylcytosine (5mC), particularly when accompanied by guanine (CpG). These modifications occur in animals and plants and can ultimately impact embryonic development, tissue regeneration, and pathogenesis.
GENEWIZ uses wet and dry lab solutions for methylation sequencing, including Methyl-Seq, to help you explore the methylome through whole genome or targeted 5-methylcytosine (5mC) detection.
Methyl-Seq Overview
After protecting modified cytosines from downstream deamination, an enzymatic step deaminates cytosine but does not affect 5caC and 5ghmC. The resulting converted sequence can be used to compare treated and untreated samples, creating a snapshot of 5mC methylation patterns across the whole genome or targeted regions of interest.
ENZYMATIC CONVERSION & SEQUENCING
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1. Protection/Enzymatic Conversion
Submit cells, tissue, or gDNA for enzymatic conversion of unprotected cytosine
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2. Library Preparation
Add adapters to ssDNA fragments
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3. Sequencing
Sequence 2×150 bp on Illumina® platform
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4. Data Analysis
Identify 5mC sites, calculate methylation ratios, and perform differential methylation analysis
Methylation Sequencing Features & Benefits
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Complete sequencing solutions from extraction and enzymatic conversion to data analysis
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Superior data quality: with a guarantee of ≥80% bases with ≥Q30 (>90% on average)
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Dedicated Ph.D.-level support at every step including post-delivery
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Industry-leading turnaround time as fast as 2 weeks
Technical Resources
NGS Platforms
For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. GENEWIZ does not guarantee data output or quality for sequencing-only projects.
