Amplicon-EZ

Amplicon sequencing made easy!

  • Easy on your budget – as low as $58 per sample
  • Easy to order – checkout online in minutes
  • Easy to submit samples – use a GENEWIZ drop box
  • Easy to analyze data – interactive reports with intuitive design
  • Easy on your deadline – results in 8 business days*

GENEWIZ Amplicon-EZ is a streamlined version of next generation sequencing to analyze heterogeneous PCR products. Our standardized workflow simplifies ordering, processing, and analysis to deliver high-quality results fast.

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Amplicon Sequencing in 8 Days*

  • 1. Sample submission

    No minimum sample number
    Convenient sample submission via dropboxes

  • 2. Library Preparation

    150-500 bp products accepted
    Submit with or without lllumina® partial adapters

  • 3. Sequencing

    -50,000 reads per sample
    Full sequence coverage with 2×250 bp sequencing

  • 4. Data Analysis

    Interactive variant report
    Unique sequence identification and abundance analysis

*Samples are processed at our Leipzig and Oxford lab facilities in cycles starting on Wednesdays with data returned 8 business days later (on the second Monday).

Applications: CRISPR SCREENING & MORE

  • CRISPR screening and validation – on/off-target mutations
  • Somatic variant analysis
  • Taxonomic identification – 16S, 18S, or ITS regions
  • Antibody discovery – variable regions of mAb, Fab, scFv, or sdAb genes
  • Clone verification and screening
  • Diversity analysis – synthetic DNA libraries

Amplicon-EZ Barcodes

Use our orange barcode labels for prepaid amplicon sequencing.

Amplicon-EZ
Data will be ready for download within 8 business days after sample receipt at GENEWIZ. orange amplicon barcode
How to Label Your Samples

Applications: CRISPR Screening & More

  • CRISPR screening and validation – on/off-target mutations
  • Somatic variant analysis
  • Taxonomic identification – 16S, 18S, or ITS regions
  • Antibody discovery – variable regions of mAb, Fab, scFv, or sdAb genes
  • Clone verification and screening
  • Diversity analysis – synthetic DNA libraries

CRISPR Screening with Amplicon-EZ

  • 1. Edit cells with CRISPR

  • 2. Amplify target region from edited cell populations

  • 3. Use Amplicon-EZ to expedite sequencing of PCR products

  • 4. Analyze results to find wells with desired mutations**

CRISPR Validation with Amplicon-EZ

  • 1. Isolate clones from positive wells (see CRISPR screening)

  • 2. Amplify target region from clones

  • 3. Use Amplicon-EZ to expedite sequencing of PCR products

  • 4. Validate edits and find homozygous mutants**

**View our interactive report (login required)

Want GENEWIZ from Azenta to perform amplification and sequencing? Learn about our additional NGS-based CRISPR validation services.

Technical Resources