Quantitative PCR (qPCR) and Digital PCR (dPCR)

 

From gene expression and genotyping to copy number variation (CNV) analysis and viral titer, GENEWIZ from Azenta offers a versatile polymerase chain reaction (PCR) portfolio that can be tailored to support the needs of your genomics project. Whether through endpoint or real-time detection, our unmatched quantitative PCR (qPCR) or digital PCR (dPCR) capabilities provide you with precise, rapid quantitation of nucleic acid sequences to simplify and advance the path toward your next breakthrough. Reach out to our experts to discuss your specific needs for nucleic acid detection.

 

PCR Services: Relative or Absolute Quantitation

Quantitative PCR (qPCR)

qPCR is the tried-and-true method for real-time detection of nucleic acid sequences, providing high-throughput analysis for speed and scalability. No matter the size of your sample concentrations, this method offers a wide detection range of target sequences. Select from convenient and affordable predeveloped assays or partner with our assay experts to design a custom assay to meet your project requirements.

Digital PCR (dPCR)

dPCR provides absolute quantitation of nucleic acid sequences without the need for complex standard curves or reference targets. Through micro reactions, dPCR is highly sensitive for increased detection of rare transcripts and low-frequency targets. This endpoint method is optimal for reproducibility and offers straightforward data analysis and interpretation of your results.


Key Applications

qPCR

  • Gene expression
  • Copy number variation (with standard curve)
  • Confirmatory testing of NGS data
  • SNP genotyping and allelic discrimination
  • Methylation analysis (CpG)
  • Pathogen detection and quality control
  • Customized qPCR solutions

dPCR

  • Absolute copy number variation (CNV)
  • Rare mutation and allelic discrimination
  • Low-abundance transcript detection
  • Viral titer (copies/mL)
  • Vector copy number (copies/genome)
  • Circulating tumor DNA (ctDNA) and cell-free DNA (cfDNA) detection in liquid biopsy

PCR for Clinical Research

Regulatory guidelines recommend the use of PCR methodology for multiple quality and safety checkpoints throughout the drug development process. For cell and gene therapy products, hyper-sensitive dPCR has largely been used for the evaluation of viral titer as well as quantification of viral vector copy number (VCN) of treated cells for AAV, lentivirus, and retrovirus.
Contact us to learn how our customized PCR solutions can help you advance your clinical research.


Features & Benefits

  • Building

    Experimental Design and Optimization

    To ensure project success
  • Building

    Assay Development and Validation

    Customized to meet your project needs
  • Building

    Standard and Customized Data Analysis

    Options available
High-throughput capacity using a single vendor for scalable PCR assays and other NGS approaches to supplement and extend your results
Ph.D.–level project managers to provide expertise in primer design, optimization, and troubleshooting
Streamline operations by integrating PCR with other GENEWIZ services and more from Azenta Life Sciences
Complete cell and gene therapy solutions including CAP-accredited and CLIA-licensed solutions to deliver high-quality cell and gene therapies

PCR Workflow

  • Building

    1. Assay/Primer Design

  • Sample Prep

    2. Sample Preparation

  • PCR Processing

    3. PCR Processing

  • Data Analysis

    4. Data Analysis & Reporting

Sample Types Accepted

Viral Particles

 

RNA or cDNA

DNA

Cell or Tissue

Technical Resources

Blog | NGS, PCR, or Sanger Sequencing: An Assay Selection Guide

This selection guide offers practical information about PCR + Sanger, qPCR, and NGS approaches to help you determine which assay best suits your project requirements, along with an interactive assay selection tool to aid your decision making.

Related Services

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Email: molgen@azenta.com| Phone: +49 (0)341 520 122-41