ハイスループットの遺伝子発現スクリーニング

次世代シークエンシングをベースとするAzenta Life Scienceのハイスループット遺伝子発現（HT-GEx）スクリーニングは、DRUG-Seqと同様、創薬と解析のための最適なアプローチです。マイクロアレイと異なり、短時間でバイアスのない3’遺伝子発現スクリーニングを提供し、標準的なRNAシークエンシングよりも抑えたコストで、細胞からデータまで、トランスクリプトームの解析を実現します。ライセートから直接解析を行うハイスループット遺伝子発現スクリーニングでは、RNAの精製が不要。時間とコストの両方を節減します。また、転写物の3’端のみ検出することで、必要なシークエンシングの深度を低減することが可能です。

Applications

Drug Discovery

Compound Treatment Phenotyping

CRISPR Treatment Phenotyping

Cell Response Screening

HIGH-THROUGHPUT GENE EXPRESSION SCREENING WORKFLOW

Technology Comparison

Metric	HT-GEx Screening	mRNA-Seq	Total RNA-Seq	Iso-Seq
Reads/Sample	~1-2M Reads	~10-30M Reads	~20-45M Reads	~1-2M Reads
Min. Sample Size	96/384	1	1	1
Cost	$	$$	$$$	$$$$
Starting Material	Cell Lysate	>20ng Total RNA	>100ng Total RNA	>1ug Total RNA
Selection Method	Poly(A) +	Poly(A) +	rRNA -	Poly(A) +
Detection Level	3' Tails of Genes	Poly(A) Transcript	All Transcript	Full-Length Poly(A) Transcript
Gene Expression
Variant Detection
Alternative Splicing
Transcriptome Assembly

Suitable method | Preferred method | Not a suitable method

Deliverables

Standard Deliverables

FASTQ files
Quality summary report

Advanced Analysis

(In addition to standard deliverables)

UMI extraction, mapping, counting, and transformation
Normalization
Differential gene expression and clustering by compound/treatment

Custom bioinformatics analysis and reports are available. Please contact us about how we can customize the analysis to answer your biological question.

Features & Benefits

Fast Turnaround Times

Starting at just 2 weeks for quick target discovery

High-Throughput Capacity

To rapidly scale up pilot or discovery projects

Superior Data Quality

Exceeding manufacturers’ benchmarks

High-throughput, cell-to-data solution for compound screening

Low-cost, unbiased coverage for use in selection of drug candidates

Receive detailed expression data for each well across multiple 96- or 384-well plates

Free technical consultation and customer support from Ph.D.-level project managers and lab scientists

Technical Resources

Webinar Series | Advancing Transcriptomics: Gene Expression Screening, Single-Cell RNA-Seq, and Beyond

With this two-part webinar series, go beyond traditional transcriptomics and learn about the various NGS approaches available for gene expression analysis. In part 1, we take an in-depth look at various gene expression approaches, including RNA-Seq, single-cell RNA-Seq, digital spatial profiling, and more. In part 2, we explore the data generated from these approaches and how they can complement each other and confirm findings.

Tech Note | Achieving Phenotypic Profiling for Rapid Drug Discovery with High-Throughput Gene Expression Screening

High-throughput technologies are critical in performing phenotypic profiling for drug discovery applications. In this tech note, Azenta Life Sciences discusses the challenges associated with traditional approaches, such as microarrays and RNA sequencing, and offers an optimized assay to achieve high-quality phenotypic profiling at a reduced cost for rapid drug discovery.

Information Sheet | High-Throughput Gene Expression (HT-GEx) Screening

Download our information sheet for a brief overview of Azenta Life Scences' high-throughput gene expression screening service.

NGS Platforms

Azenta is a certified service provider for Illumina and other cutting-edge NGS platforms. For information on our NGS platforms, as well as recommended configurations of your projects, please visit the NGS Platforms page. Azenta does not guarantee data output or quality for sequencing-only projects.

ハイスループットの遺伝子発現スクリーニング

Advancing Transcriptomics: Gene Expression Screening, Single-Cell RNA-Seq, and Beyond