The immense diversity of B-cell receptors (BCR) and T-cell receptors (TCR), generated through recombination of the variable (V), diversity (D), and joining (J) loci, is key to the adaptive immune system. Our Immuno-Profiling service couples ultra-deep sequencing of V(D)J regions and customized bioinformatics to provide comprehensive characterization of the entire BCR or TCR repertoire. In addition, recent advances in microfluidic technology allow BCR and TCR chains to be paired at single-cell resolution.

Check out our latest webinar “Advancements to the 10x Genomics Chromium™, Single-Cell RNA-Seq System” below.
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Service Options

Analyze V(D)J sequences at the RNA level using bulk or single-cell approaches.

for bulk repertoire analysis
Single-Cell Immuno-Profiling
for physical pairing of chains
BCR sequencing (VH and VL chains) Yes Yes

TCR Sequencing (α and β chains)

Yes Yes
Starting materials* Total RNA
Cell pellet
Cell suspension
V(D)J amplification method Proprietary 5' RACE Targeted enrichment of cDNA
with cell-specified barcodes
Physical pairing of VH/VL or α/β chains No Yes

*Visit our Sample Submissions Guidelines for more details.


Features & Benefits

BCR and TCR analysis available for multiple species, including human and mouse
Easy-to-interpret reports enable in-depth data analysis
Flexible sample requirements facilitate project design
Ph.D.-level consultation provides guidance before, during and after the project
Industry-leading turnaround time
Comprehensive research solutions with downstream synthetic DNA libraries available

Technical Resources

Advancements to the 10x Genomics Chromium™, Single-Cell RNA-Seq System

In this webinar, we review how recent advancements of the 10x Genomics Chromium™ system, coupled with GENEWIZ developmental efforts, can benefit the researcher. In addition, we spotlight the new Single-Cell V(D)J Solution, which can be used in conjunction with single-cell transcriptome profiling to identify pairing of full-length BCR and TCR sequences.


Example Report: Immuno-Profiling

Interactive HTML reports include the following:

  • CDR3 amino acid length distribution
  • Gene usage analysis
  • Top V(D)J combination
  • Linked CDR sequences at the nucleotide and amino acid level
  • CDR clustering analysis


Example Report: Single-Cell Immuno-Profiling

The LoupeTM Cell Browser from 10x Genomics provides interactive tools including the following:

  • Finding significant genes
  • Identifying cell types
  • Exploring substructure
  • Interated gene expression and V(D)J analysis


How To Order

Email | Phone +49 (0)341 520 122-41