Next Generation Sequencing: Amplicon-EZ

 

Amplicon sequencing made easy!

  • Easy on your budget – as low as €53/£47 per sample
  • Easy to order – checkout online in minutes
  • Easy to submit samples – use a Azenta Life Sciences drop box
  • Easy to analyze data – interactive reports with intuitive design
  • Easy on your deadline – results in 8 business days*

Amplicon-EZ is a streamlined version of next generation sequencing to analyze heterogeneous PCR products. Our standardized workflow simplifies ordering, processing, and analysis to deliver high-quality results fast. Applications include CRISPR screening, variant genotyping, antibody discovery, and much more.

To make our Amplicon-EZ service even easier and quicker for European customers, processing is now available at our European Genomics Headquarters in Leipzig, Germany.


   
 

Amplicon Sequencing in 8 Days*

1. Sample submission

  • No minimum sample number
  • Convenient sample submission via dropboxes

2. Library Preparation

  • 150-500 bp products accepted
  • Submit with or without lllumina® partial adapters

3. Sequencing

  • -50,000 reads per sample
  • Full sequence coverage with 2x2S0 bp sequencing

4. Data Analysis

  • Interactive variant report
  • Unique sequence identification and abundance analysis

*Samples are processed at our European Genomics Headquarters in Leipzig, Germany in cycles starting on Wednesdays with data returned 8 business days later (on the second Monday).

APPLICATIONS: CRISPR SCREENING & MORE


CRISPR screening and validation - on/off-target mutations

Somatic variant analysis

Taxonomic identification - 165, 185. or ITS regions

Antibody discovery – variable regions of mAb, Fab, scFv, or sdAb
genes

Clone verification and screening

Diversity analysis – synthetic DNA libraries

CRISPR Screening with Amplicon-EZ

Digital Spatial Profiling Stains

1. Edit cells with CRISPR

 
Digital Spatial Profiling Target Areas

2. Amplify target region from edited cell populations

 
Photocleave Barcodes

3. Use Amplicon-EZ to expedite sequencing of PCR products

 
Separate ROIs

4. Analyze results to find wells with desired mutations**

 

CRISPR Validation with Amplicon-EZ

Digital Spatial Profiling Stains

1. Isolate clones from postitive wells (see CRISPR screening)

 
Digital Spatial Profiling Target Areas

2. Amplify target region from clones

 
Photocleave Barcodes

3. Use Amplicon-EZ to expedite sequencing of PCR products

 
Separate ROIs

4. Validate edits and find homozygous mutants**

 

**View our interactive report (login required)


Want Azenta to perform amplification and sequencing? Learn about our additional NGS-based CRISPR validation services.

How To Order


Email | Phone +49 (0)341 520 122-41