UN-PURIFIED PCR

To streamline the submission of PCR samples for sequencing, GENEWIZ offers the added convenience of our PCR purification service. Excess primers and unincorporated dNTPs are removed from PCR sequencing templates prior to the cycle sequencing reaction via an enzymatic PCR clean-up technique. This service eliminates the need for costly and time-consuming column or gel-extraction clean ups. Let GENEWIZ remove the hassle from PCR clean-up and expedite your PCR product sequencing workflow.

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SEQUENCING FROM UN-PURIFIED PCR PRODUCTS

GENEWIZ uses a combination of enzymes to remove excess dNTPs and oligonucleotides from the PCR reaction prior to sequencing.  This approach generates a good-quality template that is ready to sequence, while avoiding common contamination problems from other, column-based purification techniques.

Enzymatic Purification Process

Technical Notes

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In order to optimize the concentration of the sequencing reaction, GENEWIZ recommends submitting a representative gel image of your PCR products along with your samples and order receipt.

This approach purifies the entire PCR reaction, and can only be used for single-band PCR products. Occasionally, certain brands of high-fidelity Taq can cause interference with the enzymatic cleanup reaction. Please contact Technical Support if you would like to discuss your specific enzyme selection.




FEATURES & BENEFITS

Time savings and cost savings - no need for time-consuming column purifications

Analysis using ABI Prism 3730xl DNA analyzers
Free GENEWIZ Universal Primers
Customized troubleshooting
Fast turnaround
>500 bases of Phred20 reads
GENEWIZ Online Ordering and Data Retrieval System - 24/7 web-based
Project design and consultation

HOW TO ORDER

*Samples must arrive at the GENEWIZ New Jersey laboratory before 10:00 am EST to qualify for Same Day service.  Note that direct-sequencing templates are not available for our Same Day service.



Email | Phone (1-877-GENEWIZ (436-3949), Ext. 2) | Live Chat