CLIA Sanger Sequencing

GENEWIZ provides CLIA Sanger sequencing as the clinical "gold standard" from our state-of-the-art CLIA-certified and CAP-accredited laboratory. This standalone sequencing service utilizes a CLIA-compliant workflow to support your clinical diagnostic assays, leading to advancements in personalized medicine and molecular diagnostics.

CLIA Sanger sequencing is best utilized as the initial readout from a PCR assay or in conjunction with next generation sequencing, specifically to elucidate complex regions not approachable by other methodologies or to confirm variants uncovered by genome-wide techniques.
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Features And Benefits

Long Sequencing Reads: DNA sequencing read lengths up to ~1000 bases

High Quality Data: Typical read will provide 800 bases Phred20
Proprietary Protocols: Best-in-class protocols to sequence difficult RNAi and GC-rich templates
Fast Turnaround Time: Results available within two to three business days

CLIA Sanger Sequencing Service Options

Pre-Mixed

For sequencing plasmids or purified PCR products, provide your template and primer mixed in the same tube for the most economical CLIA-compliant services.

Pre-Defined

If choosing our Pre-Defined Service, submit your plasmids or purified PCR products and primer in separate tubes, and a GENEWIZ scientist will mix them once your sample arrives on site.

Custom

Full-service option allows you to submit unpurified PCR product with unknown concentration along with your primer for sequencing. We will purify your PCR product as well as measure and adjust the concentration to optimize your reactions.

View our Sample Submissions Guidelines



Template Types

Purified Plasmids or PCR Products

Purified Plasmids or PCR Products

Un-Purified PCR Products

Un-Purified PCR Products

Difficult Template Sequencing

Difficult Template Sequencing

Deliverables

All customers will receive their raw data as .ab1, .seq, and .phd files along with a quality score report.


HOW TO ORDER

*Samples must arrive at the GENEWIZ New Jersey Laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.

Email | Phone (1-877-GENEWIZ (436-3949), Ext. 3350)