Note that RCA adds an additional 24 hours to the sequencing turnaround time. RCA is recommended for circular templates less than 15 kb in length.
Dean, Frank B. et al. “Rapid Amplification of Plasmid and Phage DNA Using Phi29 DNA Polymerase and Multiply-Primed Rolling Circle Amplification.” Genome Research 11.6 (2001): 1095–1099.
* Please note: Expression vectors may or may not be suitable for the RCA protocol.
**Please note: RCA is available for circular phage only; linear phage genomes will not amplify by this method.
Please visit our Sample Submission Guidelines for bacteria and phage templates for additional details.