Modified Oligo Synthesis (Oligo-Flex)

 

GENEWIZ Modified Oligo Synthesis (Oligo-Flex) offers customized oligos with a wide variety of modifications, at several scales, with HPLC or PAGE purification options. We offer various bases including locked nucleic acid (LNA), deoxyuridine(dU), deoxyinosine(dI) and backbone modifications. RNA oligos, oligo pools, ultra-pure options and custom-designed oligos with different also available. All oligos are synthesized under GENEWIZ’s strict quality control standards.  

 

Rapid COVID-19 identification
with our primers and probes


Features & Benefits

High Quality - Strict quality control system with low error rates
Advanced mass spectrometry QC available for oligo modification
Modified oligo synthesis at scales as low as 25 nmol (to minimize cost), or up to 1000 nmol
Tube or plate delivery formats available
Multiple purification methods available
Oligos are dried down for delivery, to maximize stability
Convenient ordering - online ordering system submits and manages your order in real-time
RNA oligos, oligo pools, ultra-pure options and custom-designed oligos available.

OLIGO Purification Options

Purification Applicable Length Advantages
Desalted Purification 5-90 nt Fast turnaround time and favorable price
PAGE Purification 21-150 nt
High purity >90%; suitable for long oligos
HPLC Purification 5-90 nt High purity >95%; suitable for modified oligos

Popular Oligo Modification Options

Category Modification 5' Mod 3' Mod Internal Mod 

Attachment Modification

 AminolinkerC6 - -
Thiol-C6 S-S  - -
Biotin -
 AminolinkerC12 - -
 BiotinTEG -
 Digoxigenin -
AminolinkerC7 - -
Thiol-C3 S-S - -
Cholesteryl - -
dTAminolinker - -
Quencher Modification BHQ1 - -
BHQ2 - -
Dabcyl - -
Eclipse - -
MGB - -
Fluorescent Dye Modification  FAM -
HEX - -
TAMRA -
TET - -
JOE -
ROX -
Cy5.5 -
Texas Red -
CY3 -
CY5 -
 Modified Bases dl - -
dU - -
Phosphorothioate - -
Phosphorylation  Phosphate -

Purification method: HPLC (recommended)\ PAGE

Scale: From 25nm to 1000nM

Additional chemical modifications are available; please contact Technical Support at DNAseq@genewiz.com or 908-222-0711, option 2, for further details.

Delivery Options

Tube or plate delivery formats available
Oligos are delivered in lyophilized format

Target Yield Options

GENEWIZ synthesizes primers on a per-scale basis, which refers to the initial amount of material used in synthesis. Due to the inefficiency of the coupling reactions, yields are never equal to the initial amount of starting material. Yield is typically impacted by the nature and content of the sequence. Purification and modifications will also reduce the final yield delivered.


      Length (nt)       Scale    Purification              Target Yield*           
5-90 25 nmol Desalted 5 nmol
HPLC 2 nmol
50 nmol Desalted 10 nmol
HPLC 4 nmol
PAGE 2 nmol
100 nmol Desalted 20 nmol
HPLC 8 nmol
PAGE 4 nmol
200 nmol Desalted 50 nmol
HPLC 20 nmol
PAGE 10 nmol
1 µmol Desalted 300 nmol
HPLC 120 nmol
PAGE 60 nmol
91-150 100 nmol PAGE 2 nmol
200 nmol PAGE 5 nmol
1 µmol PAGE 25 nmol

* Guaranteed yield will be determined by the exact oligo length, scale, and purification, and will be shown on the oligo order receipt. Actual yield delivered will be ±10% of guaranteed yield.

HOW TO ORDER


Place your order using our online ordering system




Email | Phone (1-877-GENEWIZ (436-3949), Ext. 2)