FuzeSeq™ Antibody Sequencing

Phage display is a powerful in vitro method for discovering high affinity antibodies in research, clinical diagnostics, and drug discovery. Historically, phage display libraries have been screened using Sanger sequencing, however, this methodology poses several limitations, such as low throughput, multiple required rounds of selection, and high false-positive rates. Today, next generation sequencing (NGS) is being incorporated into phage display workflows, offering deeper coverage and minimization of selection rounds. Yet, current NGS methodologies still pose limitations, including high cost and low data quality.

GENEWIZ’s FuzeSeq™ Antibody Sequencing applies a proprietary methodology to the next generation sequencing of phage display libraries to overcome the existing limitations of conventional NGS. FuzeSeq Antibody methodology enables sequencing of paired heavy and light variable regions, and provides higher data quality and quantity as a result.  In addition, GENEWIZ also offers custom bioinformatics for FuzeSeq Antibody projects. Our dedicated team of expert scientists are available to discuss your specific project requirements.



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Features & Benefits


Enables detection of low frequency complementarity determining regions (CDRs)


Minimizes rounds of biopanning required for the identification of high-affinity antibodies


Produces higher quality and quantity data compared to conventional Sanger and NGS methods


Facilitates quality and diversity assessment of original phage display libraries prior to selection


Pairs full-length variable heavy and light regions

FuzeSeq Antibody Workflow


To learn more about FuzeSeq Antibody Sequencing, please download our fact sheet or watch our webinar.



Sample Submission Guidelines

 GENEWIZ accepts purified phage, mixed phagemid vector DNA, and amplicons containing antibody variable heavy and light domains for FuzeSeq sequencing projects. For detailed sample submission requirements please visit our sample submission guidelines page.


Deliverables

All customers receive their raw data as FASTQ files. Unique sequence and abundance analysis are available by request.


NGS Platforms

GENEWIZ is a certified service provider for Illumina and Life Technology NGS platforms. For information on our NGS platforms as well as recommended configurations for your projects, please visit the NGS Platforms page. GENEWIZ does not guarantee data output or quality for sequencing only projects.



How To Order


Email | Phone (1-877-GENEWIZ (436-3949), Ext. 1) | Live Chat