SNP/Mutation Analysis

Characterizing Single Nucleotide Polymorphisms (SNPs) and mutations are important techniques in the molecular biologist’s toolbox.  GENEWIZ’s SNP and Mutation Analysis service helps scientists ramp up SNP screening assays and mutation detection in coding exons of interest, enabling scientists to hunt for and identify mutations of interest with speed and accuracy.
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SNP DETECTION AND MUTATION ANALYSIS

Mutations are genetic alterations that are acquired either in germ or non-germ (somatic) cells. They can contribute to various diseases and may affect the response of a patient to particular drug treatments. The mutation can be an insertion, deletion, missense or nonsense mutation in the coding or non-coding regions. In some cases, a mutation occurs at the intron-exon boundary and affects the normal splicing of the transcript.

Sanger-based mutation analysis, mutation screening, exon resequencing, and SNP discovery all involve high volumes of PCR amplification and sequencing. Whether you are using Sanger as your primary approach for SNP screening and mutation detection, or as a confirmation of NGS or microarray results, GENEWIZ can help expedite your SNP and mutation analysis assays. Utilize GENEWIZ's extensive expertise to support your SNP and mutation analysis research objectives with speed, quality, and reliability.





GENEWIZ Mutation Analysis Services

Extraction of genomic DNA from samples (fresh, fixed, and/or paraffin-embedded)
Amplicon selection and primer design to target regions of interest or SNPs
Assay development and optimization
PCR amplification of genomic DNA using optimized conditions
High-throughput PCR purification and bi-directional DNA sequencing per amplicon
Automated and manual sequencing results analysis
Final report identifying mutations

Features & Benefits

Reliable Assay Development: Our highly-trained scientists develop PCR assays targeted to your genes of interest.
High-Throughput Processing: Target genes are amplified using optimized assays; amplicons are purified and sequenced by capillary electrophoresis in our high-throughput facility. GENEWIZ can start with purified genomic DNA templates or extract genomic DNA from supplied sources.
Accurate Data Analysis: Mutations or SNP's are identified with automated and manual analysis to ensure efficiency and accuracy.  GENEWIZ can also provide customizable reports upon request.

GENEWIZ vs. In-House

Mutation Analysis Turnaround Time Comparison: GENEWIZ versus In-house

GENEWIZ TURNAROUND: 5 DAYS

IN-HOUSE TURNAROUND: 2-4 WEEKS



Deliverables

  • - Amplicon selection and primer design to target regions of interest or SNPs
  • - Assay development and optimization
  • - PCR amplification of genomic DNA using optimized conditions
  • - High-throughput PCR purification and bi-directional DNA sequencing per amplicon
  • - Automated and manual sequencing results analysis
  • - Final report identifying mutations

HOW TO ORDER

*Samples must arrive at the GENEWIZ New Jersey Laboratory before 10:00 am EST to qualify for Same Day service. Note that direct-sequencing templates are not available for our Same Day service.

Email | Phone (1-877-GENEWIZ (436-3949), Ext. 3350)