16S rRNA GENE Sequencing

A common method for identifying bacterial strains is analyzing the sequence of the gene coding for 16S ribosomal RNA (16S rRNA). 16S rRNA gene sequencing is also a standard tool for bacterial phylogenetic and taxonomic studies due to the conservation of this gene between different species of bacteria.

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16S rRNA: Let GENEWIZ Help Construct Your Phylogenies

The 16S ribosomal RNA subunit is an essential component in the 30S ribosomal complex in prokaryotes. Due to the structural importance of this gene, the pace of evolution of the gene has been relatively slow. Due to this conservation, scientists can use the 16S rRNA gene to identify prokaryotic species present in a sample. GENEWIZ sequences the 16S rRNA gene from your bacterial colonies rapidly and effectively. Simply submit your clonal bacterial sample, and GENEWIZ will perform PCR amplification and Sanger DNA sequencing using 16S primers. Save time at the bench - no bacterial gDNA extraction is required.


16S rRNA Gene Sequencing Service Details

  • PCR amplification directly from clonal bacterial colonies
  • Enzymatic purification of crude PCR product for enhanced sequencing quality
  • Additional analysis of PCR product by gel electrophoresis available by request
  • Forward and reverse sequencing of the 16S rRNA gene
  • Starting materials accepted:
    • Clonal Bacterial Colonies
    • Extracted gDNA

FEATURES & BENEFITS

Competitive Pricing
Please contact us for pricing information
High Quality
Robust 16S rRNA PCR protocols and high quality sequencing data
Fast & Reliable Turnaround
Sequencing data available within 4-6 business days
Superior Customer Service
Award-winning, easily accessible, dedicated project managers available Monday – Friday

16S rRNA GENE Sequencing

  • Starting Materials: Single Bacterial Colony


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  • PCR



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  • Sanger
    Sequencing



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Colony is picked from plate for PCR amplification.
Gene coding for 16S rRNA is amplified by PCR using gene-specific primers. Quality control: PCR product is observed by agarose gel electrophoresis.
Resulting PCR product is sequenced in both directions.

Deliverables

  • Chromatogram file (.ab1)
  • Sequencing file (.seq)
  • Agarose gel analysis available
  • Timely project updates

How to Order

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