DNA methylation silences gene expression in an epigenetic fashion by blocking transcription factor access to promoter and enhancer regions. In eukaryotes, the nucleotide cytosine is commonly methylated to form 5-methylcytosine (5mC), especially when accompanied by guanine (CpG). DNA methylation occurs in both animals and plants, playing significant roles in embryonic development, tissue regeneration, and pathogenesis.
Whole genome bisulfite sequencing (WGBS) is a robust method for global 5-methylcytosine (5mC) detection. GENEWIZ offers comprehensive wet and dry lab solutions to uncover the methylome.
Submit cells, tissue, or gDNA (untreated or bisulfite-treated)
Add adapters to ssDNA fragments (denatured by bisulfite treatment)
Sequence 2x150 bp on Illumina® platform
Identify 5mC sites, calculate methylation ratios, and perform differential methylation analysis
Sodium bisulfite treatment transforms unmethylated cytosine to uracil, which is converted to thymine upon sequencing. Sequence comparisons between treated and untreated samples create a snapshot of 5mC methylation patterns across the genome.
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