DNA Sequencing Sample Submission Guidelines


For the best Sanger sequencing results, GENEWIZ strongly recommends you follow our Sample Submission Guidelines as closely as possible. If you have any questions, our Technical Support team is here to help! 

(EU) Email: sanger.europe@azenta.com | Phone +49 (0)341 520 122-41

(UK) Email: genomics.service.uk@azenta.com | Phone +44 (0) 1279 873837


Sample Preparation 

Follow our barcode labeling instructions to avoid delays in sample processing and data delivery:

For orders with templates and primers in tubes, please use 1.5 ml flip cap reaction tubes (no screw caps). Label your tubes with barcodes according to your selected sample type to facilitate sample alignment and automated processing.

Dilute your custom sequencing primer to 10 or 5 µM (pmol/µl) according to the service type (Pre-Mixed, Pre Defined) using water. Please refer to the table below for minimum volume for each sequencing reaction.

For the amount of template required in each of our DNA Sequencing Services (Pre-Mixed, Pre-Defined), please refer to the tables below. Please make dilutions in water or Tris. For best results, do not use Tris-EDTA (TE) because EDTA will inhibit the sequencing reaction.

 



Pre-Mixed Tube or Plate

For the Pre-Mixed Service, prepare purified template (DNA) and primer in a total volume of 10µl in the same tube according to the table below. 


 Template  Primer  Template + Your Primer
DNA Type Submitted in Concentration  Volume  Concentration  Volume*

 Pre-Mixed Total Volume
Plasmids purified 1.5 ml reaction tube

30-100 ng / µl 5 µl 5 pmol/ µl (µM)

5 µl

 10  µl
PCR fragments purified 10-50 ng / µl
 Plasmid purified 96-well plate  30-100 ng / µl  5 µl  5 pmol/ µl (µM)  5 µl  10 µl
 PCR fragments purified  10-50 ng / µl

Video | Pre-Mixed Tube Ordering Guide

This Sanger ordering guide explains how to place a Pre-Mixed Tube sequencing order via your Azenta account and label samples correctly.


Pre-Defined Tube

For Pre-Defined Services, provide template (20 µl) and your primer (20 µl) in separate tubes for up to eight reactions according to the table below. 


 

Template Primer
DNA Type Submitted in Concentration Volume Submitted in Concentration Volume
Plasmid purified   1.5 ml reaction tube  30-100 ng / µl  20 µl  1.5 ml reaction tubes  10 pmol/µl (µM)  20 µl
PCR fragments purified/ unpurified   10-50 ng / µl  20 µl

 



Video | Pre-Defined Tube Ordering Guide

This Sanger ordering guide explains how to place a Pre-Defined Tube sequencing order via your Azenta account, order primers and label samples correctly.


Pre-Defined Vario Plate

For Pre-Defined Sanger Sequencing service in 96-well plates, provide 20 µl template per well and for primer provide separately 60 µl per plate. 


 

Template Primer
DNA Type Submitted in Concentration Volume Submitted in Concentration Your Primer Volume Per Plate
Plasmid purified 96-well plate  30-100 ng/µl 20 µl 1.5 ml reaction tubes

10 pmol/µl

(µM)

20 µl - for up to 8 reactions

60 µl - for more than 8 reactions

PCR fragments Purified/ Unpurified 10-50 ng / µl 20 µl



Video | Pre-Defined Vario Plate Ordering Guide

This Sanger ordering guide explains how to place a Vario Plate sequencing order via your Azenta account, order primers and label samples correctly.


Pre-Defined Plate

For Pre-Defined Sanger Sequencing service in 96-well plates, provide 20 µl template per well and for primer provide separately 60 µl per plate. 



 Template  Primer
DNA Type Submitted in Concentration Volume Submitted in Concentration Your Primer Volume Per Plate
Plasmid purified 96-well plate

30-100 ng/µl 20 µl 1.5 ml reaction tube

10 pmol/µl (µM)
60 µl

PCR fragments purified / unpurified 10-50 ng/µl
 
20 µl

 

GENEWIZ SANGER Sequencing Logistic Options


GENEWIZ offers convenient logistic options for sample pick up or shipment to our European sequencing labs. Learn more about logistic options available.

 

HOW TO ORDER


Email EU: sanger.europe@genewiz.com | Phone: +49 (0)341 520 122-41

   Email UK: dnasequk@genewiz.com | Phone: +44 (0) 1279 873837