PREMIUM Sanger SERVICES

GENEWIZ offers full-service Sanger sequencing options, allowing researchers to hand off an entire sequencing project. We'll manage your project from primer design to variance analysis and report generation. Our template discovery service options include primer walking, sequencing confirmation, and colony screening.

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PRIMER WALKING

Is your freezer full of unmapped or mystery constructs? Do your transformations grow on the wrong antibiotic resistance plate? GENEWIZ Primer Walking service is designed for situations when you need to determine the sequence of a completely unknown plasmid or PCR product.

Our Service Includes

  • Primer design and synthesis 
  • Plasmid preparation or RCA (if needed) 
  • Single-stranded or double-stranded coverage per base pair as dictated by project requirements 
  • Manual editing based on sequence chromatogram 
  • Sequence data assembly for consensus sequence 
  • Consensus report

Upon Order Submission

Please send to GENEWIZ:

  • Template, in tubes or plates
  • Known sequence starting point, such as antibiotic resistance, M13 promoter, etc.
  • Requested number of base pairs sequenced
  • Single-stranded or double-stranded coverage requirements

Project Deliverables

  • Standard turnaround time starting at 1 – 2 kb/week
  • Consensus sequence, in .seq or .fasta format
  • All raw sequencing data, in .ab1 and .seq formats
  • Any size plasmid prep, upon request

SEQUENCE CONFIRMATION SERVICE

Do your digestions show incorrect banding patterns? Does your construct fail to sequence or amplify with the anticipated primer set? GENEWIZ Sequence Confirmation service is designed for situations when you need to verify the identity of your construct with single- or double-stranded certainty.

Our Service Includes

  • Primer design and synthesis  
  • Plasmid preparation or RCA (if needed)
  • Single-stranded or double-stranded coverage per base pairs as dictated by project requirements 
  • Manual editing based on sequence chromatogram 
  • Sequence data assembly for consensus sequence 
  • Consensus report

Upon Order Submission

Please send to GENEWIZ:

  • Template, in tubes or plates
  • Reference sequence, in .fasta, .txt. or .seq format
  • Requested number of base pairs sequenced
  • Single-stranded or double-stranded coverage requirements


Project Deliverables

  • Standard turnaround time starting at 5-7 business days
  • Consensus sequence, in .seq or .fasta format
  • Variance report, if applicable
  • All raw sequencing data, in .ab1 and .seq formats
  • Any size plasmid prep, upon request

HOW TO ORDER


Email: sanger.europe@genewiz.com | Phone:  +49 (0)341 520 122-41