Concentration

20,000 units/ml

Recognition Site

5’...G↓AATTC...3’

3’...CTTAA↑G...5’

Applications

Genomic DNA, plasmid DNA, PCR product

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Storage Buffer

20 mM Tris-HCl pH 7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol

10× FlyCut® Buffer

500 mM Tris-Ac pH 7.9, 1 M KAc, 120 mM MgAc₂, 1 mg/ml BSA

Storage

-20°C for two years

Shipping Condition

Dry ice (-70°C)