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FlyCut® EcoRI

FlyCut® EcoRI is expressed and purified from E.coli that carries the recombinant EcoRI gene. The molecular weight is 32.0 kDa, with the recognition site at G^AATTC. The reaction is conducted for 5-15 minutes at 37°C, and heat-inactivated at 65°C for 20 minutes. This enzyme is not sensitive to dam, dcm methylation, but sensitive to mammalian CpG methylation.

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Concentration

20,000 units/ml

Recognition Site

5’...G↓AATTC...3’

3’...CTTAA↑G...5’

Applications

Genomic DNA, plasmid DNA, PCR product

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 μg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 μl.

Storage Buffer

20 mM Tris-HCl pH 7.4, 250 mM NaCl, 0.1 mM EDTA, 1.5 mM DTT, 400 μg/ml BSA, 50% Glycerol

10× FlyCut® Buffer

500 mM Tris-Ac pH 7.9, 1 M KAc, 120 mM MgAc2, 1 mg/ml BSA

Storage

-20°C for two years

Shipping Condition

Dry ice (-70°C)

 

Kit Contents

Component JE201-01 JE201-02
FlyCut® EcoRI  5,000 units  2 × 5,000 units 
10× FlyCut® Buffer 2 × 1 ml 3 × 1 ml
10× DNA Loading  Buffer 1 ml 1 ml