TransStart® FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase. TransStart® FastPfuFly DNA Polymerase has an extension rate of up to 6 kb/min. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has higher extension rate, higher fidelity, and higher amplification efficiency.
• TransStart® FastPfu Fly DNA Polymerase offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 2-6 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.
Applications
• High fidelity PCR
• High yield and fast PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Product Contents
|
Component |
AP231-01/11 |
AP231-02/12 |
|
TransStart® FastPfu Fly DNA Polymerase |
250 U×1 |
500 U×1 |
|
5×TransStart® FastPfu Fly Buffer |
1.2 ml×1 |
1.2 ml ×2 |
|
2.5 mM dNTPs |
- / 500 μl×1 |
- / 1 ml ×1 |
|
50 mM MgSO4 |
200 μl×1 |
400 μl ×1 |
|
PCR Stimulant |
200 μl×1 |
400 μl×1 |
|
6×DNA Loading Buffer |
500 μl×1 |
1 ml ×1 |
US
Europe (English)
Chinese
Japanese