TransStart® FastPfu Fly PCR SuperMix is a ready-to-use mixture of TransStart® FastPfu Fly DNA polymerase, dNTPs, and optimized buffer. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2O.
• TransStart® FastPfu Fly PCR SuperMix offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
• Extension rate is about 2-6 kb/min.
• PCR products can be directly cloned into pEASY®-Blunt vectors.
• Amplification of genomic DNA fragment up to 15 kb.
• Amplification of plasmid DNA fragment up to 20 kb.
Applications
• High fidelity PCR
• High yield PCR
• GC-rich PCR
• Blunt end cloning
• Site-directed mutagenesis
• Complex templates
Storage
at -20 ℃ for two years
Shipping
Dry ice (-70 ℃)
Product Contents
Component |
AS231-01 |
AS231-02 |
2×TransStart® FastPfu Fly PCR SuperMix (-dye) |
1 ml |
5×1 ml |
Nuclease-free Water |
1 ml |
5 ml |