Highlights |
• Extension rate is about 1-2 kb/min. |
Applications |
• Routine PCR |
Unit Definition |
One unit of EasyTaq® DNA Polymerase incorporates 10 nmol of deoxyribonucleotide into acid-precipitable material in 30 minutes at 74°C. |
Quality Control |
• Functional absence of double- and single-strand endonuclease activity; >99% homogeneous measured by SDS-PAGE. • Each batch of EasyTaq® DNA Polymerase has been assayed for amplification efficiency of the p53 gene from 10 ng of human genomic DNA. |
Storage Buffer |
20 mM Tris-HCl (pH 8.0), 0.1 mM EDTA, 1 mM DTT, 100 mM KCl, 50% glycerol, stabilizers. |
Storage |
-20°C for two years |
Kit Contents
Component |
AP111-01 |
AP111-02 |
AP111-03 |
AP111-04 |
EasyTaq® DNA Polymerase |
500 U×1 |
500 U×6 |
2500 U×4 |
5000 U×10 |
10×EasyTaq® Buffer |
1.2 ml×1 |
1.2 ml×6 |
1.2 ml×20 |
1.2 ml×100 |
6×DNA Loading Buffer |
1 ml×1 |
1 ml×2 |
1 ml×4 |
1 ml×20 |