RNAi Template and GC-rich Template Sequencing

RNAi Template Sequencing -

RNA Interference (RNAi) refers to the mechanism by which double stranded RNA (dsRNA) specifically suppresses the expression of a gene bearing its complementary sequence. It is an emerging technology with successful applications for quick and definitive studies of gene functions.

RNAi template sequencing, however, can often present challenges due to its double-stranded or "hairpin" structures. The small hairpin RNA (shRNA) templates used in RNAi studies frequently cannot be successfully sequenced, due to early termination or ambiguity. GENEWIZ has developed effective proprietary techniques for sequencing shRNA templates. Our proprietary RNAi Protocol is producing superior results for our customers. For comparison, see the graph below:

GC-Rich Sequencing -

GENEWIZ proprietary protocol has been proven successful in sequencing templates with high GC-content and templates with other prohibitive secondary structures.

How to Order -

You can choose one of the two service types - Pre-Mixed or Custom. When filling out sequencing instructions, select one of our proprietary protocols listed in the "Special Protocol" column. Additional charges apply. GENEWIZ customer support is always available to discuss your project with you.