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Site-Directed Mutagenesis

GENEWIZ can increase your research efficiency by performing site-directed mutagenesis, including deletion, insertion, and point mutations. Our specialized services enable you to obtain mutant constructs quickly.


  • Features & Benefits
  • Service Overview
  • Applications

Features & Benefits


  • Fast Turnaround Time: Delivery of the sequence-confirmed mutant as miniprep DNA from five working days
  • 100% Accuracy: Optimized GENEWIZ protocol for greater reliability; High-fidelity enzymes to minimize PCR errors; Sequence the mutated region to ensure 100% accuracy
  • Consultation & Support: Ph.D. scientists with over 12 years of DNA sequencing and molecular biology experience are available to answer questions and discuss your projects
  • Customer-oriented Project Management: You will receive weekly project updates as your order moves towards completion.
  • Competitive, reaction-based pricing

Site-directed Mutagenesis services provide reliable solutions for your mutagenesis project needs. GENEWIZ helps increase your research productivity by performing time-consuming site-directed mutagenesis projects efficiently, with fast in-house sequence confirmation.

Service Overview


  • Any form of single, multiple (substitutions, insertions, deletions, or truncations) or random mutations
  • Any size or number of mutations/variants
  • Gene Synthesis service for multiple challenging mutations/variants

Site-Directed Mutagenesis Service Process:


  • Design and synthesis of mutagenesis primers
  • PCR amplification
  • Confirmation of desired mutations by DNA sequencing
  • Delivery of purified plasmid DNA construct or its glycerol stock

Applications


  • Study protein function, structure-function relationships, and nucleic acid-protein interactions
  • Identify active sites or functional elements such as promoter regions
  • Engineer novel proteins for drug discovery
  • Construct fusion proteins, insert or delete tags, or generate site or domain knockouts
  • Modify regulatory sequences to alter gene expression

How To Order


  1. Get a quote by completing the online quotation form:

  2. GENEWIZ will contact you within one business day to discuss your project in detail.

Please contact us for more information. GENEWIZ Project Management is available to discuss your project from 8:00 a.m. - 8:00 p.m. (ET), Monday - Friday.