Q: Why use GENEWIZ gene synthesis services?
Gene synthesis is an efficient and cost-effective alternative to molecular cloning for custom gene production. When compared to PCR cloning, GENEWIZ gene synthesis offers faster turnaround time for a comparable price. For a comparison of GENEWIZ custom gene synthesis services versus traditional molecular cloning, please click here.
Q: How does GENEWIZ synthesize genes?
Single-stranded oligonucleotides are designed, synthesized, and assembled using different protocols depending on sequence characteristics. Subsequently, the assembled full-length gene is cloned into the pUC57 vector; the insert is verified by Sanger DNA sequencing and restriction digestion.
Q: What gene length can GENEWIZ synthesize?
GENEWIZ will synthesize cloned gene sequences ranging from 1 bp to 50+ kb, using short DNA oligos as building blocks. For standard priority gene synthesis, products delivered as purified PCR fragments are limited to 200 bp to 1.2 kb.
Q: Can GENEWIZ synthesize sequences with high GC content or repeats?
GENEWIZ has a proven track record of synthesizing difficult templates, such as GC-rich sequences and sequences with repeats, using proprietary technologies. With our bioinformatics platform, GENEWIZ optimizes your gene for synthesis. In some very rare instances, extreme sequence content may be refractory to available sequence verification techniques. GENEWIZ carefully reviews every sequence to estimate difficulty and anticipate project challenges, and customizes every project quotation to include an estimated turnaround time and pricing information based on this analysis.
Q: How long does it take to synthesize a gene?
*Applicable to non-complex genes delivered in a standard pUC57 vector.
Q: Are there any additional fees beyond the per bp price?
GENEWIZ provides a custom quote for every gene synthesis project. For standard priority, typical gene sequences (non-repetitive, average GC-content) that do not require custom subcloning, no additional fees apply. For genes with expedited handling, difficult sequences, large inserts, or for projects with custom subcloning requirements, any relevant fees will be indicated in your custom gene synthesis quote.
Q: Can GENEWIZ optimize genes?
GENEWIZ can deliver codon-optimized genes, designed to meet your exact research specifications.
Q: How does codon optimization enhance gene synthesis projects?
GENEWIZ codon optimization tool has the ability to optimize multiple critical parameters to stabilize DNA fragments and improve gene expression efficiency. Parameters include but are not limited to:
Q: What vector does GENEWIZ clone into?
The assembled full-length gene is cloned into the pUC57 vector, and verified with Sanger DNA sequencing on at least one strand and restriction digestion.For standard priority gene synthesis, products between 200 bp and 1.2 kb may also be delivered as a purified PCR fragment, and are verified by Sanger DNA sequencing on both strands.
Q: What restriction sites does GENEWIZ use when cloning synthetic genes into
Q: How do I request a gene synthesis quote and place an order?
To request a gene synthesis quote, please click here to complete the simple online quote request form. GENEWIZ will contact you within one business day to discuss your project in detail.
Q: How do I request a TurboGENETM quote and place an order?
To request a TurboGENETM quote, please click here to complete the simple online quote request form. GENEWIZ will contact you within one business day to discuss your project in detail.
Q: Who provides assistance with gene synthesis projects?
GENEWIZ Project Management is composed of specialized experts dedicated to your project's success. Project Management is available to assist with any questions throughout the duration of your gene synthesis project. In addition, GENEWIZ Project Management will provide milestone updates at each stage of your gene synthesis process, keeping you well-informed throughout all stages of research.