Q: I am a new customer, how do I set up an account?
On our homepage (www.genewiz.com) you will see a "Register" link to set up a new account in the upper right-hand corner. Clicking on this link will allow you to set up your login name (your e-mail address) and password. This process takes only a few minutes; then you will receive an account activation e-mail. Simply click on the link in this e-mail to activate your account. You can now login to your account using your e-mail address and the password you just created. You are ready to submit your first order!
Q: How can I try out your service?
Q: What is the price of my order?
For DNA sequencing, price display during the order submission process is available for some accounts. If this is a feature you are interested in activating, just contact your GENEWIZ Sales Executive to find out if it is available for your institution. You can also contact your Sales Executive for a quotation. One more option is to contact GENEWIZ after you have submitted your order, to obtain the price for that specific order.
Q: Can I open an account and submit samples if I live outside the US?
Absolutely! We welcome customers from all over the world. When you set up a new online account, you will be asked for your state. Living outside the USA, choose “other” from the drop down menu. Please provide the correct city and postal code. All of the other fields are filled out as designated.
Q: Why should I choose GENEWIZ?
GENEWIZ is an operational excellence company with a customer-driven strategy. We have become the leader in DNA sequencing and molecular biology services, including gene synthesis, by providing the best combination of value - fast turnaround, specialized expertise, competitive prices, and friendly customer support. We continuously innovate to produce the best results with the greatest convenience for our customers.
Q: What is the difference between Pre-Mixed, Pre-Defined, and Custom?
Choose "Pre-Mixed" if you have adjusted your sample concentration following our guidelines and have already added your own sequencing primers, or are requesting one of GENEWIZ’s free universal primers.
Q: How do I prepare my samples so I will get good DNA sequencing results?
While the majority of reactions sequence well, you should avoid several situations that can create unfavorable results. Here are guidelines to help you optimize sequencing results:
Q: What types of DNA templates can be sequenced?
Q: How much DNA and primer should I submit?
Please closely follow the DNA Sequencing Sample Submission Guidelines.
Q: How do I determine my DNA concentration?
We recommend that you check your DNA concentration and associated 260/280 and 260/230 ratios with a spectrophotometer such as a NanoDrop. You can also estimate your DNA concentration on an agarose gel by loading a small amount of DNA and running it with a marker of known concentration.
Q: Can I use 8 strip tubes if I am submitting less than 8 samples?
Yes, you can just cut off the unused tubes and use them for your next submission, or send the entire 8-strip with labels on just the tubes containing your samples.
Q: Can you sequence very short (100 bp) PCR?
We highly recommend that you sequence PCR products that are no shorter than 200 bp (500 bp or more is ideal).
Q: What universal primers do you have?
We provide these free universal primers:
Q: Can you help me if I my sequence is GC-rich and hard to amplify?
Yes, GENEWIZ has proprietary protocols that have proven successful in sequencing templates with high GC content, as well as templates with other prohibitive secondary structures such as hairpins. When filling out sequencing instructions, select one of our proprietary protocols listed in the "Special Protocol" column. Additional charges apply.
Q: How long do you keep my samples?
We keep your DNA samples for one week, unless otherwise specified. If you need either your DNA or your primers for future orders, please specify this in the “Comments” section of your order. Just write “Please keep my DNA, primer, or both for future orders.” We can store your DNA for one month or your primers for up to a year.
Q: How do I know if my vector has a binding site for a universal primer?
Check your vector map or manufacturer’s protocol. It will tell you what primer site is present or what primer is recommended for sequencing. You can then compare this to the GENEWIZ universal primers. Simply click on the “GENEWIZ Free Universal Primer” link and you will see the free universal primers supplied by GENEWIZ with their respective sequence information.
Q: What do I need to provide for you to perform PCR cleanup on my samples?
Submit your unpurified PCR products with your primer(s) in separate, well-labeled tubes. Also include a gel image and the amount loaded on the gel. Be sure to select “Custom” as your service type and choose “PCR clean up” from the Special Request column when submitting your order. We will perform the clean up and then optimize the sequencing reaction based on your gel image.
Q: What type of 96-well plate should I use to submit my samples in bulk format?
Feel free to use any brand of PCR plates and caps. Be sure to load your plates in the column or vertical direction. Here are a few suggestions of plate/cap combinations that work well for a tight seal:
Q: How should I label my 8-strip tubes?
Label your tube on the SIDE with the tube ID generated from your online order form. The tube ID is made up of your initials and the tube number. See figure below (If GENEWIZ was submitting samples, the tube ID would be GW01-GW08-as labeled below):
Q: When will I receive my DNA sequencing results?
If you requested Same Day service, you will have your results within 7 hours of samples arriving at GENEWIZ (when samples are received by 10 AM). If you requested Standard Service, you will have your results by noon the next business day following sample receipt at GENEWIZ. Note that customers using our San Diego, Boston and Maryland Labs enjoy the Faster Local Service. Please contact us for details and eligibility. We always send an e-mail notification when your results are available. Feel free to login to your account at any time to check the status of your order.
Q: What software can I use to view my results?
From the “Recent Results” section of your account, you have access to the page where you can download your data (both the .seq and .ab1 files). The .seq file is a text file and can be opened with any program that can view text files (Wordpad, Notepad, etc.) If you are having difficulty opening these files, you can change the file extension from .seq to .txt as a last resort to view it.
Q: How long do my results stay in the online system?
We strongly recommend that you download all of your results and back them up on your laboratory computer. Our current policy is to store your results for 6 months (please note that any data older than 3 months will require transfer back from our archive and can take up to 24 hours after initiating the transfer request from your results page). This policy is subject to change at anytime, but changes, if any, will be clearly communicated.
Q: How do I design primers for sequencing?
We recommend designing your sequencing primers in a region that is 100 bases upstream of your sequence of interest. If you do not have the luxury of having this buffer, the closest you want the primer to be to your area of interest is 50-60 bases. Anything closer and you risk missing a portion of your area of interest. The primers should be about 18-24 bases in length with a Tm of 56-60 degrees. The GC content should be about 45-55%. Many vendors that provide oligo synthesis services have software into which you can plug your primer sequence to check for Tm, GC content and homodimerization. For the oligo purity, desalting is all that is needed for sequencing.
Q: Can you design and order primers for me?
Yes, we can design and order primers for you; an additional charge applies. If you already have the primer sequence, you will simply fill out the “Sequencing Primer” order form. If you would like GENEWIZ to design the primer for you, please send the reference sequence with your area of interest highlighted or otherwise indicated to firstname.lastname@example.org.
Q: What should I do if my sequencing results failed?
Q: What does it mean when I have a poly A/T region resulting in poor quality
Q: Are you GLP (Good Laboratory Practice) or cGMP compliant?
GENEWIZ’s Quality System is developed in accordance with applicable FDA regulations described in Title 21, Part 58 of the Code of Federal Regulations for Good Laboratory Practice. In addition, our quality system includes elements of cGMP compliance (21CFR211, 600, ICH Q7A) to support biopharmaceutical manufacturing Quality Control testing. We offer GLP services including GLP DNA sequencing and GLP level molecular biology projects. For more details, please contact us at 877-GENEWIZ (436-3949) or Regulatory@genewiz.com.