RNA interference (RNAi), a hugely important technological breakthrough in modern biomedical research, is widely used in target identification and validation. The vector-based shRNA approach has become increasingly attractive for researchers utilizing RNAi technology, due to the ease of intracellular delivery and its sustainable expression characteristics.

The construction of such shRNA plasmids, however, is a long and laborious process, and involves dealing with multiple vendors including oligo synthesis, DNA sequencing, large-scale and transfection-grade plasmid preparation, etc. Scientists are often additionally faced with challenges in shRNA synthesis, amplification of small hairpin fragments, and sequence verification of the difficult small hairpin region - all of which cause significant project delays or failures. GENEWIZ offers a complete package of shRNA cloning services.

Your Benefits Include

• Customized cloning strategy based on hairpin length
• Reliable shRNA oligo synthesis and amplification
• Most effective proprietary shRNA sequencing protocol in industry
• Great time and cost savings through efficient workflow integration from oligo synthesis to transfection-ready plasmid DNA preparation

You give us the sequences, and we deliver the plasmids ready for transfection!
Please contact us for more information. GENEWIZ Project Management is always available to discuss your project with you.