Single- and Double-Stranded Primer Walking Services

GENEWIZ can help you obtain sequence information of an unknown clone, or confirm the sequence integrity of your clone. Our primer walking services will deliver the confirmed sequence information you need.

This service includes -

• Primer design and synthesis
• DNA template preparation if needed
• Single-stranded or double-stranded sequencing as per project requirement
• Manual editing based on sequence chromatogram
• Sequence data assembly
• Consensus report and discrepancy report if applicable (File format: .SEQ, .GB/.GBK, or MS WORD)

How to order and submit samples

To order primer walking services, please download Primer Walking Service Order Form and email your order to dnaseq@genewiz.com. Also print out a hard copy of the order form and send in with your samples.

Plasmid: Submit 1 µg per kb of target sequence for single stranded coverage and 2 µg per kb sequence for double stranded coverage. Submit at least 10 µg plasmid if region of interest is of unknown length.

Primers: GENEWIZ can synthesize initial primer for you if the primer sequence is provided. If you have primers that can be used in the initial round of sequencing, please submit at least 10 µl each at 5 µM.

Packaging: GENEWIZ recommends 1.5-ml screw cap tubes for DNA and primer submission. Please label the tubes clearly with sample/primer name and concentration. Tighten the tube caps and use enough cushion in the package. Please ship your package to GENEWIZ with standard overnight service or contact us at dnaseq@genewiz.com to find out if there is a GENEWIZ sample drop box near your location.