DNA Sequencing - DNA and Primer Pre-Mixed by Users or Sequencing with GENEWIZ Primers (Pre-Mixed)

This service is ideal for sequencing of plasmid DNA and PCR products. Follow our sample preparation guidelines to pre-adjust your DNA concentration and add your primer or have GENEWIZ add one of our free universal vector primers.

Your benefits include -

• Analysis using ABI Prism 3730xl DNA analyzers
• Read lengths approximately 1000 bases and > 700 bases of Phred20 reads
• Free universal vector primers
• On-line order submission and GENEWIZ CLIMS sample tracking
• 24/7 web-based data retrieval
• GENEWIZ customer support is always available to discuss your project with you

Turnaround time -

• Standard Service - Data reported within one business day after samples are delivered to GENEWIZ, guaranteed!
• Same Day Service - Data reported by 5pm EST the same day samples are delivered to GENEWIZ (by 10 am EST). Up to 94 samples per order, in most cases an additional charge applies. Note: For San Diego customers we offer a local service that is next day from the day we pick up the samples. Same Day service for San Diego is coming soon!  Log in or create a GENEWIZ account to get San Diego service information.

Please place your order on-line. If you have any questions about submitting your samples, please feel free to contact technical support at dnaseq@genewiz.com.

Pre-Mixed Sample Preparation Guidelines

DNA Type DNA Size DNA Volume (Total Amount) Primer Volume (Total Amount) Plasmid < 6 kb 8 µl (~400 ng) 4 µl (8 pmol) Plasmid 6 - 10 kb 8 µl (~600 ng) 4 µl (8 pmol) Plasmid > 10 kb 8 µl (~800 ng) 4 µl (8 pmol) Purified PCR* 200 - 500 bp 8 µl (3 - 10 ng) 4 µl (8 pmol) Purified PCR* 0.5 - 1 kb 8 µl (5 - 20 ng) 4 µl (8 pmol) Purified PCR* 1-2 kb 8 µl (10 - 40 ng) 4 µl (8 pmol)

*PCR products should be purified by either gel extraction or enzymatic purification. Pre-mix your DNA template and primer in an 8-strip 0.2 ml PCR tube, with a total volume of 12 µl. Submit 8 µl of DNA when using GENEWIZ's Free Universal Primers.

Please label your tube on the SIDE with the tube ID generated from your online order form. Cap your 8-strip tubes tightly with matching 8-strip caps. Please provide some cushion around the tubes to avoid any potential damage during shipping.

If submitting samples in 96-well plate format, please use column-loading scheme (see the diagram below) and leave two wells for GENEWIZ controls. Seal the plates with caps and provide some cushion to avoid potential damage during shipping.