DNA Sequencing - DNA and Primer Submitted Separately with Concentrations Adjusted by Users (Pre-Defined)

This service applies to sequencing of plasmid DNA and PCR products. Follow our sample preparation guidelines to pre-adjust your DNA concentration. GENEWIZ will add your primer or one of our free universal vector primers to each reaction. In most cases, a small additional charge applies.

Your benefits include -

• Analysis using ABI Prism 3730xl DNA analyzers
• Mixing of DNA and primer by GENEWIZ technical staff
• Read lengths approximately 1000 bases and> 700 bases of Phred20 reads
• Free universal vector primers
• On-line order submission and GENEWIZ CLIMS sample tracking
• 24/7 web-based data retrieval
• GENEWIZ customer support is always available to discuss your project

Turnaround time -

• Standard Service - Data reported within one business day after samples are delivered to GENEWIZ, guaranteed!
• Same Day Service - Data reported by 5pm EST the day samples are delivered to GENEWIZ (by 10 am EST). Up to 94 samples per order, in most cases a small additional charge applies. Note: For San Diego customers we offer a local service that is next day from the day we pick up the samples. Same Day service for San Diego is coming soon!  Log in or create a GENEWIZ account to get San Diego service information.

Please place your order on-line. If you have any questions about submitting your samples, please feel free to contact technical support at dnaseq@genewiz.com.

Pre-Defined Sample Preparation Guidelines -

Submit your template and primer separately in 8-strip 0.2ml PCR tubes.
Primer Concentration should be at 5 pmol/µl with a minimum volume of 5 µl,
add 5 µl of primer for each additional sequencing reaction.

DNA Type DNA Size DNA Conc. in H2O Minimum DNA Volume (Total Amount) DS plasmid < 6 kb 40 - 60 ng/µl 8 µl (~400 ng) DS plasmid 6 - 10 kb 60 - 80 ng/µl 8 µl (~600 ng) DS plasmid > 10 kb 80 - 100 ng/µl 8 µl (~800 ng) Purified PCR* 200 - 500 bp ~1 ng/µl 8 µl (3 - 10 ng) Purified PCR* 0.5 - 1 kb ~2 ng/µl 8 µl (5 - 20 ng) Purified PCR* 1 - 2 kb ~4 ng/µl 8 µl (10 - 40 ng)

*PCR products should be purified by either gel extraction or enzymatic purification.

Please label both the DNA and primer tubes. Label your DNA tube on the SIDE with the tube ID generated from your online order form. Cap your 8-strip tubes tightly with matching 8-strip caps. Please provide some cushion around the tubes to avoid any potential damage during shipping.

If submitting samples in 96-well plate format, please use column-loading scheme (see the diagram below) and leave two wells for GENEWIZ controls. Seal the plates with caps and provide some cushion to avoid potential damage during shipping.