Microsatellite (STR) Analysis

Microsatellite marker, also known as short tandem repeats (STRs), are polymorphic DNA loci consisting of a repeated nucleotide sequence, ranged from 2 to 7 base pairs in length. The number of repeats is highly variable in a population, thereby creating multiple alleles for a given locus. Microsatellite analysis is commonly used for genetic mapping, linkage analysis and to trace inheritance patterns. GENEWIZ offers a complete package of microsatellite analysis.

This service includes -

• Microsatellite assay development, including amplicon selection and primer design
• Assay optimization and validation
• PCR amplification of genomic DNA using validated conditions
• Electrophoresis of PCR amplicons using ABI3730xl
• Analysis of fragment size and allele calling

Please provide detailed information regarding the size range of each allele of a locus. An assay can include multiplex PCRs with primers that are fluorescently labeled. If you are planning to develop your own assay, we recommend using the following dye set: 6FAM, VIC, NED and PET. Microsatellite analysis is performed on our ABI3730xl DNA Analyzer, a capillary electrophoresis platform that generates more accurate size calling, consistent band intensities and low run-to-run migration variations.

To order this service, please email dnaseq@genewiz.com with your project information. GENEWIZ Customer Support will contact you to discuss the project scope in detail.