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DNA Sequencing - DNA and Primer Submitted Separately with Concentrations
Adjusted by GENEWIZ (Custom)
Use custom DNA sequencing if you want GENEWIZ to check the
quality and quantity of your DNA samples. Please submit your DNA templates and
sequencing primers separately, using your choice of sample tubes. In most cases
a small additional charge applies.
Your benefits include -
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One business day turnaround, guaranteed
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Template quantity and quality check with Nanodrop
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Mixing of DNA and primer by GENEWIZ technical staff
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Analysis using ABI Prism 3730xl
DNA analyzers
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Read lengths approximately 1000 bases and > 700 bases of Phred20 reads
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Free universal vector primers
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On-line order submission and GENEWIZ CLIMS sample tracking
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24/7 web-based data retrieval
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GENEWIZ customer support is always available to discuss your project with you
Please place your order
on-line. If you have any questions about submitting your
samples, please feel free to contact technical support at
dnaseq@genewiz.com.
Sample Preparation -
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Submit your DNA in an 8-strip 0.2 ml PCR tube (please feel free to use 1.5 ml eppendorf tubes if you are submitting one DNA sample with many primers). Make sure that your tubes are clearly marked with either the tube label or the sample name.
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Please submit 500 -800 ng or more of plasmid DNA, or 80-150 ng or more of PCR
fragment for each sequencing reaction
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Sequencing primer (5 µl per reaction) should be submitted as 5 pmol/µl (5 µM)
in dH20. The primer can be submitted in 0.5 ml or 1.5 ml eppendorff
tubes with the primer name and concentration clearly labeled.
PCR Clean-up (Performed by GENEWIZ) -
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If you have a single PCR band and you would like GENEWIZ to
perform the PCR clean up, you can select Custom Sequencing and choose PCR
Clean-up from the Special Protocol column. We also request that you submit a
gel image of your PCR product with the amount loaded in each well clearly
labeled.
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If you would like to perform the PCR clean up, we highly
recommend using the ExoSAP-IT kit from USB Scientific (Cat. #78200).
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