GENEWIZ can help you obtain sequence information of an unknown clone, or confirm the sequence integrity of your clone. Our primer walking services will deliver the sequence information you need.

Your Benefits Include

• Primer design and synthesis
• Plasmid preparation if needed
• Single-stranded or double-stranded sequencing as per project requirements
• Manual editing based on sequence chromatogram
• Sequence data assembly for consensus sequence
• Consensus report and discrepancy report if applicable

How To Order

Please login and place your order online. Print your order receipt and include it with your samples.

Sample Submission Details

• Plasmid - Submit 1 µg per kb of target sequence for single stranded coverage and 2 µg per kb sequence for double stranded coverage. Submit at least 10 µg plasmid if region of interest is of unknown length.
• Primers - GENEWIZ can synthesize the initial primer for you if the primer sequence is provided. If you have primers that can be used in the initial round of sequencing, please submit a minimum of 10 µl each at 5 µM.
• Packaging - For DNA and primers, please use 1.5 ml screw cap tubes and label them with sample or primer name and concentration.
• Shipping - Send samples to GENEWIZ via Standard Overnight service or contact us at mb@genewiz.com to find out if there is a GENEWIZ Drop Box or courier pickup service at your location.

Standard Deliverables

• Consensus report and discrepancies, if applicable (.doc)
• Consensus sequence (.seq)
• Sequencing data (.ab1)