Gene Synthesis Speeds Up Cloning Projects
Are you frustrated with the slow pace of your traditional cloning project? Try gene synthesis!
Compare traditional cloning with GENEWIZ's Gene Synthesis Service to see why gene synthesis is a better choice:
| Traditional Cloning | Gene Synthesis at GENEWIZ |
|---|---|
| Lengthy, Complex Procedure: | Short, Simple Procedure: |
|
1. Cloning strategy design 2. Primer Synthesis 3. PCR and restriction enzyme digestion 4. Gel extraction and ligation 5. Transformation and plasmid miniprep 6. Screen by enzymatic digestion 7. Sequencing reaction preparation 8. Sequence assembly analysis Troubleshoot if any of the above steps fail |
1. You provide the sequence 2. You receive the gene in your choice of vector, ready for the next experiment |
| Challenges: | Advantages: |
|
× Requires a physical template × Can make only one or a few changes × Labor intensive × Unpredictable results × Troubleshooting drains your reagents and time |
√ No physical template
needed √ Codon optimize or custom design your gene √ Reliable delivery by GENEWIZ Ph.D. experts √ Project turnaround starts from 2 weeks |
